LOCUS       sequence-102009-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' Gateway Entry Vector Containing QUAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons 
            MJ, Halpern ME
  TITLE     Adoption of the Q transcriptional regulatory system for zebrafish 
            transgenesis.
  JOURNAL   Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. 
            Epub 2013 Jun 20.
  PUBMED    23792917
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2013.06.012"; journalName: "Methods"; date: 
            "2014-04-1- 1"; volume: "66"; issue: "3"; pages: "433-40"
COMMENT     Sequence Label: p5E-QUAS (L4-R1)
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..91
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     83..100
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     84..100
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    140..235
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    427..550
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(622..641)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(623..641)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(646..662)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(646..662)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(764..783)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(842..861)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
       61 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggcccgagtt
      121 aacgctacca tggagctcca aataatgatt ttattttgac tgatagtgac ctgttcgttg
      181 caacaaattg ataagcaatg cttttttata atgccaactt tgtatagaaa agttggggta
      241 atcgcttatc ctcggataaa caattatcct cacgggtaat cgcttatccg ctcgggtaat
      301 cgcttatcct cgggtaatcg cttatccttg caagggtcga ctctagaggg tatataatgg
      361 atcccatcgc gtctcagcct cactttgagc tcctccacac gaattccctc gacctcgaag
      421 acgcgtcaag tttgtacaaa aaagttgaac gagaaacgta aaatgatata aatatcaata
      481 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc
      541 agtcactatg aaccaactac ttagatggta ttagtgacct gtagaattcg agctctagag
      601 ctgcagggcg gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
      661 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
      721 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
      781 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
      841 gggtataaat gggctcgcga taa
//
