LOCUS       sequence-102012-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes an inert blue fluorescent protein for use in oxidizing 
            cellular environments including the periplasm and mammalian 
            secretory pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Costantini LM, Subach OM, Jaureguiberry-bravo M, Verkhusha VV, Snapp
            EL
  TITLE     Cysteineless non-glycosylated monomeric blue fluorescent protein, 
            secBFP2, for studies in the eukaryotic secretory pathway.
  JOURNAL   Biochem Biophys Res Commun. 2013 Jan 18;430(3):1114-9. doi: 
            10.1016/j.bbrc.2012.12.028. Epub 2012 Dec 19.
  PUBMED    23257162
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2012.12.028"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-01-18- 18"; volume: "430"; issue: "3"; pages: 
            "1114-9"
COMMENT     Sequence Label: psecBFP2
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..81
                     /label=MCS
                     /note="multiple cloning site"
     CDS             98..793
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVDNHHFKATSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFIDHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGSLIYNVKIRGVDFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANDETYVEQHEVAVARYSDL
                     PSKLGHKLN"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
       61 gcgggcccgg gatccaccgg tcgccaccat gagcgaggag ctgattaagg agaacatgca
      121 catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaaggcaa catccgaggg
      181 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
      241 tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat
      301 cgaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg
      361 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
      421 ccaggacggc agcctcatct acaacgtcaa gatcagaggg gtggacttca catccaacgg
      481 ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc
      541 tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct
      601 gatcgcaaac gccaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc
      661 tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaacg acgagaccta
      721 cgtcgagcag cacgaggtgg cagtggccag atacagcgac ctccctagca aactggggca
      781 caagcttaat taagcggccg cg
//
