LOCUS       sequence-102014-        2702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mutant of hamster BiP-mGFP unable to bind clients..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Lai CW, Aronson DE, Snapp EL
  TITLE     BiP availability distinguishes states of homeostasis and stress in 
            the endoplasmic reticulum of living cells.
  JOURNAL   Mol Biol Cell. 2010 Jun 15;21(12):1909-21. doi: 
            10.1091/mbc.E09-12-1066. Epub 2010 Apr 21.
  PUBMED    20410136
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-12-1066"; journalName: "Mol Biol Cell"; date: 
            "2010-06-15- 15"; volume: "21"; issue: "12"; pages: "1909-21"
COMMENT     Sequence Label: BiP-mGFP P495L
FEATURES             Location/Qualifiers
     source          1..2702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1960..1969
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1966..1968,1969..1971,1972..2682)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2011..2032)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2272..2291)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2619..2640
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgaagttcc ctatggtggc ggcggcgctg ctgctgctct gcgcggtgcg ggccgaggag
       61 gaggacaaga aggaggatgt gggcacggtg gtcggcatcg acctggggac cacctattcc
      121 tgcgttggtg tgttcaagaa cggccgcgtg gagatcatag ccaacgatca gggcaaccgc
      181 atcacgccgt cgtatgtggc cttcactcct gaaggcgagc gtctgattgg cgatgcggcc
      241 aagaaccagc tcacctccaa tcccgagaac acggtcttcg acgccaagcg cctcatcgga
      301 cgcacttgga atgacccttc agtgcagcag gacatcaagt tcttgccttt caaggtggtt
      361 gaaaagaaaa ctaaaccata cattcaagtt gatattggag gtgggcaaac caaaacattt
      421 gccccagaag aaatttctgc catggttctc actaaaatga aagaaactgc tgaagcatat
      481 ttgggaaaga aggttaccca tgcagttgtt actgtgccgg cttacttcaa tgatgcccag
      541 cgccaagcaa ccaaagatgc tggcaccatt gctggactga atgtcatgcg gatcatcaat
      601 gagcccacag cagctgctat tgcgtatggc ctggataaga gagagggcga gaagaacatc
      661 ctcgtttttg acctgggcgg tggaaccttc gatgtgtctc ttctgaccat tgacaatggt
      721 gtctttgaag tggtggccac gaatggagac actcatctcg gtggggaaga ctttgatcag
      781 cgggttatgg aacacttcat caagctgtac aaaaagaaaa ctgggaaaga cgttagaaaa
      841 gacaacagag ctgtgcagaa acttcgtcgt gaggtggaaa aggctaagcg agccctgtct
      901 tctcagcatc aagcaagaat tgagatagag tccttctttg aaggagaaga cttctctgag
      961 accctgactc gggccaaatt tgaagagttg aacatggacc tgttccgatc taccatgaag
     1021 ccagtccaga aagtgttgga agactctgat ctgaagaaat cagacattga tgaaattgtt
     1081 cttgtcggtg ggtctactcg gattcccaag attcagcagc tggtgaaaga gttcttcaat
     1141 ggcaaggagc catcccgtgg cataaaccca gatgaggctg tagcatacgg tgctgctgtc
     1201 caggctggtg tcctctctgg tgatcaagat acaggtgatc tggtactgct tgatgtatgt
     1261 cctcttacac ttggtattga aacagtggga ggtgtcatga ccaaactgat tccaaggaac
     1321 actgtggtac ccaccaagaa gtctcagatc ttttccacag cttctgataa tcagccaact
     1381 gtaacaatca aggtctatga aggtgaacga cccctaacaa aagacaacca tcttctgggt
     1441 acatttgatc tgactggaat tcctcctgct cctcgtgggg tattccagat tgaagtcacc
     1501 tttgagatag atgttaatgg tattcttcga gtgacagctg aagacaaagg tacagggaac
     1561 aaaaacaaaa tcacaattac caatgaccaa aatcgcctga cacctgaaga aattgaaagg
     1621 atggttaatg atgcagagaa gtttgctgag gaagacaaaa agctcaaaga gcgcattgat
     1681 accaggaacg agttggaaag ctatgcttac tctctcaaga accagattgg agataaagaa
     1741 aagctgggcg gtaaactttc ctctgaagat aaagaaacca tggagaaagc tgtagaggaa
     1801 aagattgaat ggctggaaag ccaccaggat gcagacattg aagactttaa agctaaaaag
     1861 aaggaactag aggaaattgt tcagcctatt attagcaaac tctatggaag tgcaggccct
     1921 cccccaactg gtgaagagga tacatcagaa ccaccggtcg ccaccatggt gagcaagggc
     1981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2041 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2161 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2221 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2281 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2401 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     2461 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2521 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2581 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2641 accgccgccg ggatcactct cggcatggac gagctgtaca aggatgaatt gtaagcggcc
     2701 gc
//
