LOCUS       sequence-102023-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QNS
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(457..475)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      471..518
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(693..698,699..715,716..721))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(748..766)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggccataaag aagttcaatt gaaagatcaa attttaggtg ttctggatta tttagaaaaa
      121 caacagtctg catggccatt tcttaagcca gttagtcttt cagaagctcc tgattattat
      181 gatattataa aagaaccaac agatattttg accatgagaa gaaaggccag acatggtgat
      241 tataaaacga aggaagattt tggtattgag cttaagagaa tgtttgataa ttgtcgtttg
      301 tataatgctc caacaactat ttatttcaaa tatgcaaatg aactacaaac acttatatgg
      361 cctaaatatg aagctatatg atgacgaagc ttgcggccgc actcgaggat ccggctgcta
      421 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      481 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      541 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
      601 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
      661 gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca
      721 aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
      781 tcatg
//
