LOCUS       sequence-102025-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QNS
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(358..378)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(391..408)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             418..440
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    455..479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(479..498)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(480..498)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(582..601)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agcagccggg atcctcgagt gcggccgcaa gcttcgtcat catatagctt catatttagg
       61 ccatataagt gtttgtagtt catttgcata tttgaaataa atagttgttg gagcattata
      121 caaacgacaa ttatcaaaca ttctcttaag ctcaatacca aaatcttcct tcgttttata
      181 atcaccatgt ctggcctttc ttctcatggt caaaatatct gttggttctt ttataatatc
      241 ataataatca ggagcttctg aaagactaac tggcttaaga aatggccatg cagactgttg
      301 tttttctaaa taatccagaa cacctaaaat ttgatctttc aattgaactt ctttatggcc
      361 ctggaaatac aagttttctc tgccgctgct gtgatgatga tgatgatgca tggtatatct
      421 ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc
      481 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat
      541 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
      601 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
      661 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
      721 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
      781 gc
//
