LOCUS       sequence-102032-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human USP21 (USP domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Ye Y, Akutsu M, Reyes-Turcu F, Enchev RI, Wilkinson KD, Komander D
  TITLE     Polyubiquitin binding and cross-reactivity in the USP domain 
            deubiquitinase USP21.
  JOURNAL   EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.1038/embor.2011.17. Epub 
            2011 Mar 11.
  PUBMED    21399617
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/embor.2011.17"; journalName: "EMBO Rep"; date: "2011-04"; 
            volume: "12"; issue: "4"; pages: "350-7"
COMMENT     Sequence Label: pOPINS-USP21 (USP, aa 196-565)
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..91
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    106..245
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(171..190)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     331..353
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(347..538)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
       61 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
      121 tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc
      181 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
      241 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
      301 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
      361 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg
      421 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
      481 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
      541 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga
      601 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
      661 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
      721 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc
      781 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacg
//
