LOCUS       sequence-102033-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses vOTU (OTU domain from CCHFV) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Akutsu M, Ye Y, Virdee S, Chin JW, Komander D
  TITLE     Molecular basis for ubiquitin and ISG15 cross-reactivity in viral 
            ovarian tumor domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2228-33. doi: 
            10.1073/pnas.1015287108. Epub 2011 Jan 25.
  PUBMED    21266548
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1015287108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-02-8- 8"; volume: "108"; issue: "6"; pages: "2228-33"
COMMENT     Sequence Label: pOPINK-vOTU (CCHFV OTU, aa 1-183)
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..91
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    106..245
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(171..190)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     331..353
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(347..538)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
       61 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
      121 tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc
      181 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
      241 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
      301 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
      361 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg
      421 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
      481 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
      541 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga
      601 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
      661 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
      721 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc
      781 agatccggaa cataatggtg cagggcgctg acttccgcg
//
