LOCUS       sequence-102059-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB122
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     75..94
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(190..468)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 tattgcaatg aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat
       61 gatgtcccca taatttttgg cagagggaaa aagatctcag tggtatttgt gagccagggc
      121 attggccaca ccagccacca ccttctgata ggcagcctgc acctgagtgt ttacttgtac
      181 ttatctagtc tttgagattc gtcggaacac atgataatag aggtcccagg cttggaggag
      241 gtccttgaca ttccctgatt tcatgtactt cctgcaccac tcttgggcct ccattaaatc
      301 tcgaccatag gcctgattaa aggatgtttc cttcagagtc tgggggcccc gttccatcat
      361 agcatgcaag ggctccagca cctcaaacat gcctttcacg ttcctttccc caaagtacaa
      421 acgagatgcc tcttccaggc cttcatgcca catctcatgc cagaggattc tagatccggt
      481 ggatcccggg cccgcggtac ccttgtacag ctcgtccatg ccgccggtgg agtggcggcc
      541 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa
      601 cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
      661 cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc
      721 cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt
      781 cttctgcatt ac
//
