LOCUS       sequence-102062-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive miRempty_Mis12-GFP-FKBP3; inducible mCh-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB111
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             271..549
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     primer_bind     complement(645..664)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    710..765
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(764..783)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
       61 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      121 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      181 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagggt
      241 accgcgggcc cgggatccac cggatctaga atcctctggc atgagatgtg gcatgaaggc
      301 ctggaagagg catctcgttt gtactttggg gaaaggaacg tgaaaggcat gtttgaggtg
      361 ctggagccct tgcatgctat gatggaacgg ggcccccaga ctctgaagga aacatccttt
      421 aatcaggcct atggtcgaga tttaatggag gcccaagagt ggtgcaggaa gtacatgaaa
      481 tcagggaatg tcaaggacct cctccaagcc tgggacctct attatcatgt gttccgacga
      541 atctcaaaga ctagataagt acaagtaaac actcaggtgc aggctgccta tcagaaggtg
      601 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
      661 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
      721 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
      781 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
      841 g
//
