LOCUS       sequence-102065-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^549-718.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB174
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(681..700)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     ncRNA           complement(775..816)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 gctcaccatg gtggcgactg ctagcataga tattttcagt cgtttcgatt ccttttccac
       61 attgtctcta atgttctgta gttttctgga gttctgaacc agggatacta aactctccct
      121 aaaatcacta gtcccatgat ctctgccaac attatgaagc tcatcaaaga aagtcaacgt
      181 ctgtttgagt ttggcctgaa caattttttg ctcttctaat tctgcaagaa gggcctgctt
      241 agtacataat tcagtcttgt acttctcctg taactgttca atttctttct ggagatgctg
      301 aaaatcttcc tcactataag gtgtctcctt acatttatct tcaggaagca agatgtttga
      361 gggaatacgt aaaatcagct gcaaaaacag ttgctccatt ttgctaaaaa ggttatcaaa
      421 atgtcctttc atgaagcaaa gaaacttctc tgtgcatttg cgaatctgca ctgggctaat
      481 gtcacagtct gggatgccat ccagcttctt cagaataacc tgttcaacgg cctgcatcac
      541 ttcaaatagg tagtcttgaa atgcaatgta gatccgaagc atgcacgttt gtggcgtgaa
      601 gccaaagaac tgggcctcgt aggtcattgg atccacagac atggtggcgc gccggtggat
      661 cccgggcccg cggtaccgac acctgtggag agaaaggcaa agtggatgtc agtaagacca
      721 ataggtgcct atctggtcca cgcgtatgcg atatcagcag ctagcgccga attcggccat
      781 ttgttccatg tgagtgctag taacaggcct tgtgtcctga tatggattca tgcacatggt
      841 cagtcagt
//
