LOCUS       sequence-102067-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-584 (MID).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB161
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(689..708)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 tcgcccttgc tcaccatggt ggcgactgct agcatagata ttttcagtcg tttcgattcc
       61 ttttccacat tgtctctaat gttctgtagt tttctggagt tctgaaccag ggatactaaa
      121 ctctccctaa aatcactagt cccatgatct ctgccaacat tatgaagctc atcaaagaaa
      181 gtcaacgtct gtttgagttt ggcctgaaca attttttgct cttctaattc tgcaagaagg
      241 gcctgcttag tacataattc agtcttgtac ttctcctgta actgttcaat ttctttctgg
      301 agatgctgaa aatcttcctc actataaggt gtctccttac atttatcttc aggaagcaag
      361 atgtttgagg gaatacgtaa aatcagctgc aaaaacagtt gctccatttt gctaaaaagg
      421 ttatcaaaat gtcctttcat gaagcaaaga aacttctctg tgcatttgcg aatctgcact
      481 gggctaatgt cacagtctgg gatgccatcc agcttcttca gaataacctg ttcaacggcc
      541 tgcatcactt caaataggta gtcttgaaat gcaatgtaga tccgaagcat gcacgtttgt
      601 ggcgtgaagc caaagaactg ggcctcgtag gtcattggat ccacagacat ggtggcgcgc
      661 cggtggatcc cgggcccgcg gtaccgacac ctgtggagag aaaggcaaag tggatgtcag
      721 taagaccaat aggtgcctat ctggtccacg cgtatgcgat atcagcagct agcg
//
