LOCUS       sequence-102068-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-584 (MID).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB161
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..330
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             337..657
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             337..657
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ttctctagag gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
       61 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
      121 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
      181 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
      241 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
      301 gtcttcgatg tggagcttct aaaactggaa actagaggcg tgcaggtcga gaccatcagc
      361 cccggcgacg gccgcacctt tcccaagaga ggccagactt gcgtggtcca ctacaccggc
      421 atgctggagg acggcaagaa gttcgacagc agccgcgacc gcaacaagcc cttcaagttc
      481 atgctgggca aacaggaagt gatccgcggc tgggaggaag gcgtggctca gatgagcgtg
      541 gggcagcggg ccaagctgac catcagcccc gactatgcct acggcgccac cggccacccc
      601 ggcatcatcc ccccccacgc caccctggtg ttcgacgtgg agctgctgaa gctggagact
      661 agtgaattcg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
      721 cgcggccaga cctgcgtggt gcactaca
//
