LOCUS       sequence-102070-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-718-AAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB172
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..337
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             344..664
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             344..664
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 tggtgaattc tctagaggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt
       61 ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa
      121 atttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt
      181 gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac
      241 tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc
      301 cactctcgtc ttcgatgtgg agcttctaaa actggaaact agaggcgtgc aggtcgagac
      361 catcagcccc ggcgacggcc gcacctttcc caagagaggc cagacttgcg tggtccacta
      421 caccggcatg ctggaggacg gcaagaagtt cgacagcagc cgcgaccgca acaagccctt
      481 caagttcatg ctgggcaaac aggaagtgat ccgcggctgg gaggaaggcg tggctcagat
      541 gagcgtgggg cagcgggcca agctgaccat cagccccgac tatgcctacg gcgccaccgg
      601 ccaccccggc atcatccccc cccacgccac cctggtgttc gacgtggagc tgctgaagct
      661 ggagactagt gaattcggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt
      721 ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag at
//
