LOCUS       sequence-102072-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-718.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB171
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(135..413)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     primer_bind     complement(460..479)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     ncRNA           complement(554..595)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(621..647)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
       61 aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct caccatggtg
      121 gcgaccggac tagtctttga gattcgtcgg aacacatgat aatagaggtc ccaggcttgg
      181 aggaggtcct tgacattccc tgatttcatg tacttcctgc accactcttg ggcctccatt
      241 aaatctcgac cataggcctg attaaaggat gtttccttca gagtctgggg gccccgttcc
      301 atcatagcat gcaagggctc cagcacctca aacatgcctt tcacgttcct ttccccaaag
      361 tacaaacgag atgcctcttc caggccttca tgccacatct catgccagag gattctagaa
      421 gccatggtga ccggtggatc ccgggcccgc ggtaccgaca cctgtggaga gaaaggcaaa
      481 gtggatgtca gtaagaccaa taggtgccta tctggtccac gcgtatgcga tatcagcagc
      541 tagcgccgaa ttcggccatt tgttccatgt gagtgctagt aacaggcctt gtgtcctgtg
      601 agacgcatct gtacgtctct agcatacagc cttcagcaag cctccagagc tctagaagtc
      661 gaccgaaacg caagagtctt ctctgtctcg acaagcccag tttctattgg tctccttaaa
      721 cctgtcttgt aaccttgata cttacctgcc cagtgcctca cgaccaactt ctgc
//
