LOCUS       sequence-102081-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB127
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..328
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             335..655
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             335..655
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ctctagagga gtgcaggtgg aaaccatctc cccaggagac gggcgcacct tccccaagcg
       61 cggccagacc tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga aatttgattc
      121 ctcccgggac agaaacaagc cctttaagtt tatgctaggc aagcaggagg tgatccgagg
      181 ctgggaagaa ggggttgccc agatgagtgt gggtcagaga gccaaactga ctatatctcc
      241 agattatgcc tatggtgcca ctgggcaccc aggcatcatc ccaccacatg ccactctcgt
      301 cttcgatgtg gagcttctaa aactggaaac tagaggcgtg caggtcgaga ccatcagccc
      361 cggcgacggc cgcacctttc ccaagagagg ccagacttgc gtggtccact acaccggcat
      421 gctggaggac ggcaagaagt tcgacagcag ccgcgaccgc aacaagccct tcaagttcat
      481 gctgggcaaa caggaagtga tccgcggctg ggaggaaggc gtggctcaga tgagcgtggg
      541 gcagcgggcc aagctgacca tcagccccga ctatgcctac ggcgccaccg gccaccccgg
      601 catcatcccc ccccacgcca ccctggtgtt cgacgtggag ctgctgaagc tggagactag
      661 tgaattcgga gtgca
//
