LOCUS       sequence-102084-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Const. Halotag-GFP-PACT + mCherry-DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Ballister ER, Aonbangkhen C, Mayo AM, Lampson MA, Chenoweth DM
  TITLE     Localized light-induced protein dimerization in living cells using a
            photocaged dimerizer.
  JOURNAL   Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475.
  PUBMED    25400104
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-17"; volume: "5:5475. doi"; pages: " 
            10.1038/ncomms6475"
COMMENT     Sequence Label: pERB219
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             256..729
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             730..759
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
       61 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      121 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      181 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtataaggcc
      241 ggcctggagt ctagaatcag tctgattgcg gcgttagcgg tagatcgcgt tatcggcatg
      301 gaaaacgcca tgccgtggaa cctgcctgcc gatctcgcct ggtttaaacg caacacctta
      361 aataaacccg tgattatggg ccgccatacc tgggaatcaa tcggtcgtcc gttgccagga
      421 cgcaaaaata ttatcctcag cagtcaaccg ggtacggacg atcgcgtaac gtgggtgaag
      481 tcggtggatg aagccatcgc ggcgtgtggt gacgtaccag aaatcatggt gattggcggc
      541 ggtcgcgttt atgaacagtt cttgccaaaa gcgcaaaaac tgtatctgac gcatatcgac
      601 gcagaagtgg aaggcgacac ccatttcccg gattacgagc cggatgactg ggaatcggta
      661 ttcagcgaat tccacgatgc tgatgcgcag aactctcaca gctattgctt tgagattctg
      721 gagcggcggg aacaaaaact catctcagaa gaggatcttt aattaagcgc tcgaggcggc
      781 cgcaattcac tcctcaggtg caggctgcct atca
//
