LOCUS       sequence-102087-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Const. Halotag-GFP-Mito + mCherry-DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Ballister ER, Aonbangkhen C, Mayo AM, Lampson MA, Chenoweth DM
  TITLE     Localized light-induced protein dimerization in living cells using a
            photocaged dimerizer.
  JOURNAL   Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475.
  PUBMED    25400104
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-17"; volume: "5:5475. doi"; pages: " 
            10.1038/ncomms6475"
COMMENT     Sequence Label: pERB217
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(428..445)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     655..674
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tggtgaatcc ggtgatgcat cgggaagtgg aagtggatct cgagctcaag cttcgaattc
       61 aaaactaatt gctaaaagtg cagaagacga aaaagcgaag gaagaaccag ggaaccatag
      121 gatcgtaatt cttgcaatgt tagctattgg cgtgttctct ttaggggcgc ttatcaaaat
      181 tattcaatta agaaaaaata attaaggatc caccggatct agataactga tctccggatg
      241 acgataagct taaggatccg cccctctccc tccccccccc ctaacgttac tggccgaagc
      301 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
      361 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
      421 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
      481 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
      541 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
      601 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
      661 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
      721 tctgatctgg ggcctcggta cacatgc
//
