LOCUS       sequence-102088-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused to LINuS, a light-inducible nuclear localization 
            signal (biNLS2/IkB-alpha NES).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di 
            Ventura B
  TITLE     Engineering light-inducible nuclear localization signals for precise
            spatiotemporal control of protein dynamics in living cells.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.
  PUBMED    25019686
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4404. doi"; pages: " 
            10.1038/ncomms5404"
COMMENT     Sequence Label: pDN77
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             156..860
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(298..316)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     566..585
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcat gcccagcacc cggatccagc
      121 agcagctggg ccagctgacc ctggagaacc tgcaggtgag caagggcgag gaggataaca
      181 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      241 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      301 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      361 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      421 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      481 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      541 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      601 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
      661 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
      721 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
      781 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
      841 gcatggacga gctgtacaag ggtggatctg gaggttcagg tggaagtttg gctactacac
      901 ttgaacgtat tgagaagaac tttgtcatta ctgacccaag attgccagat aatcccatta
      961 tattcgcgtc cgatagtttc ttgcagttga cagaatatag ccgtgaagaa attttgggaa
     1021 gaaactgcag gtttctacaa ggtcctgaa
//
