LOCUS       sequence-102094-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused to LINuS, a light-inducible nuclear localization 
            signal (biNLS2/IkB-alpha NES).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di 
            Ventura B
  TITLE     Engineering light-inducible nuclear localization signals for precise
            spatiotemporal control of protein dynamics in living cells.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.
  PUBMED    25019686
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4404. doi"; pages: " 
            10.1038/ncomms5404"
COMMENT     Sequence Label: pDN77
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..86
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(966..985)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
       61 ctggcaacta gaaggcacag tcgaggctga tcagcggttt aaacttaagc ttggtaccga
      121 gctcggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg
      181 ctcgagctag tccagctttt tcttcttggc tgcggctgcc aggttggcgt cgggcagtct
      241 ctttgcggcc tcgtcaatat tttctgcagt tttcttaatc agcatgactc cctctctctc
      301 ggcagcatct cggacatgct cagttccatc caactgaacc ccaataaagt actggacatc
      361 tcccttctga tctcgcatag gctgcaagtg aaagaggttc cagaactttt taccactctt
      421 tgtataatta atcagctgaa cagtgacctc tgtttggtta tctatggcat ctctaatttt
      481 tctcactgtc gcgcgatcag tttcaggacc ttgtagaaac ctgcagtttc ttcccaaaat
      541 ttcttcacgg ctatattctg tcaactgcaa gaaactatcg gacgcgaata taatgggatt
      601 atctggcaat cttgggtcag taatgacaaa gttcttctca atacgttcaa gtgtagtagc
      661 caaacttcca cctgaacctc cagatccacc cttgtacagc tcgtccatgc cgccggtgga
      721 gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt
      781 gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt
      841 gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt
      901 gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc
      961 catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac
     1021 cttgtagatg aactcgccgt cctgcaggga ggagtcctgg
//
