LOCUS       sequence-102099-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Eiteneuer A, Seiler J, Weith M, Beullens M, Lesage B, Krenn V, 
            Musacchio A, Bollen M, Meyer H
  TITLE     Inhibitor-3 ensures bipolar mitotic spindle attachment by limiting 
            association of SDS22 with kinetochore-bound protein phosphatase-1.
  JOURNAL   EMBO J. 2014 Oct 8. pii: e201489054.
  PUBMED    25298395
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2014 Oct 8. pii: e201489054."
COMMENT     Sequence Label: pcDNA5FRT/TO-GFP-I3(V41A/W43A)
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(165..167,168..170,171..683)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(210..231)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(471..490)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt caccatggtg agcaagggcg
      181 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      241 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      301 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      361 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      421 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      481 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      541 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      601 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
      661 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      721 acacccccat cggcgacggc cccgtgctgc tg
//
