LOCUS       sequence-10211-e       10037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10037)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 10037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pQCXIH CMV/TO SV40 Large T K1 mutant (w417-1)
FEATURES             Location/Qualifiers
     source          1..10037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1609..1912
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1913..2116
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2118..2136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2139..2157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2198..2222
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2259..4385
                     /codon_start=1
                     /product="large T antigen from SV40 (simian virus 40)"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSKEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     protein_bind    complement(4400..4424)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4477..4518
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(4566..5140,5141..5143,5144..5152)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4733..4750)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             5178..6197
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5178..6197
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     LTR             6743..7168
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7271..7293)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7429..7449)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7434..7763
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7614..7749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7785..7804
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7885..7902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8056..8644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8136..8155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8815..9606,9607..9675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9438..9457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9676..9780)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9848..9866
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9966..10037,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg ataagcttgg gagttccgcg ttacataact
     1621 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     1681 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     1741 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     1801 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     1861 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     1921 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     1981 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     2041 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     2101 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
     2161 tcgactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca aaaaagcagg
     2221 ctttaaagga accaattcag tcgactggat ccaccggtat ggataaagtt ttaaacagag
     2281 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgcctgg gggaatattc
     2341 ctctgatgag aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat aaaggaggag
     2401 atgaagaaaa aatgaagaaa atgaatactc tgtacaagaa aatggaagat ggagtaaaat
     2461 atgctcatca acctgacttt ggaggcttct gggatgcaac tgagattcca acctatggaa
     2521 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcaaaag
     2581 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     2641 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     2701 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     2761 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     2821 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     2881 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     2941 aggaatattt gatgtatagt gccttgacta gagatccatt ttctgttatt gaggaaagtt
     3001 tgccaggtgg gttaaaggag catgatttta atccagaaga agcagaggaa actaaacaag
     3061 tgtcctggaa gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat
     3121 tgcttgggat gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa
     3181 aagaacagcc cagccactat aagtaccatg aaaagcatta tgcaaatgct gctatatttg
     3241 ctgacagcaa aaaccaaaaa accatatgcc aacaggctgt tgatactgtt ttagctaaaa
     3301 agcgggttga tagcctacaa ttaactagag aacaaatgtt aacaaacaga tttaatgatc
     3361 ttttggatag gatggatata atgtttggtt ctacaggctc tgctgacata gaagaatgga
     3421 tggctggagt tgcttggcta cactgtttgt tgcccaaaat ggattcagtg gtgtatgact
     3481 ttttaaaatg catggtgtac aacattccta aaaaaagata ctggctgttt aaaggaccaa
     3541 ttgatagtgg taaaactaca ttagcagctg ctttgcttga attatgtggg gggaaagctt
     3601 taaatgttaa tttgcccttg gacaggctga actttgagct aggagtagct attgaccagt
     3661 ttttagtagt ttttgaggat gtaaagggca ctggagggga gtccagagat ttgccttcag
     3721 gtcagggaat taataacctg gacaatttaa gggattattt ggatggcagt gttaaggtaa
     3781 acttagaaaa gaaacaccta aataaaagaa ctcaaatatt tccccctgga atagtcacca
     3841 tgaatgagta cagtgtgcct aaaacactgc aggccagatt tgtaaaacaa atagatttta
     3901 ggcccaaaga ttatttaaag cattgcctgg aacgcagtga gtttttgtta gaaaagagaa
     3961 taattcaaag tggcattgct ttgcttctta tgttaatttg gtacagacct gtggctgagt
     4021 ttgctcaaag tattcagagc agaattgtgg agtggaaaga gagattggac aaagagttta
     4081 gtttgtcagt gtatcaaaaa atgaagttta atgtggctat gggaattgga gttttagatt
     4141 ggctaagaaa cagtgatgat gatgatgaag acagccagga aaatgctgat aaaaatgaag
     4201 atggtgggga gaagaacatg gaagactcag ggcatgaaac aggcattgat tcacagtccc
     4261 aaggctcatt tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc
     4321 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4381 cataagtcga gatatctaga cccagctttc ttgtacaaag tggttgatat ccagcacagt
     4441 ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
     4501 ctcggtctcg attctacgcg taccggtagg cctcgtacgc ttaattaacc ggatccggaa
     4561 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     4621 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     4681 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     4741 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     4801 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     4861 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     4921 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtagtc
     4981 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggaatctg atctggggcc
     5041 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac
     5101 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccgtaccaa
     5161 agatggatag atccggaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     5221 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     5281 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     5341 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     5401 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     5461 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     5521 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     5581 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     5641 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     5701 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     5761 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     5821 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     5881 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     5941 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     6001 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     6061 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     6121 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     6181 cgagggcaaa ggaatagagt agatgccgac cgaacaagag ctgatttcga gaacgcctca
     6241 gccagcaact cgcgcgagcc tagcaaggca aatgcgagag aacggcctta cgcttggtgg
     6301 cacagttctc gtccacagtt cgctaagctc gctcggctgg gtcgcgggag ggccggtcgc
     6361 agtgattcag gcccttctgg attgtgttgg tccccagggc acgattgtca tgcccacgca
     6421 ctcgggtgat ctgactgatc ccgcagattg gagatcgccg cccgtgcctg ccgattgggt
     6481 gcagatctag tcgagggctg cagcgctgca gaggccgagt gcagaactgc tccaaaggga
     6541 cctcaaggct ttccgaggga cactaggctg actccatcga gccagtgtag agataagctt
     6601 atcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc
     6661 aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa aagattttat
     6721 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     6781 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat
     6841 caaggtcagg aacagatgga acagggtcga ccctagagaa ccatcagatg tttccagggt
     6901 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     6961 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     7021 ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
     7081 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
     7141 gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc
     7201 ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg
     7261 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     7321 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     7381 cagccatgac ccagtcacgt agcgatagcg gagtgtagat ccggctgtgg aatgtgtgtc
     7441 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7501 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7561 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7621 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7681 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7741 ttggaggcct aggcttttgc aaaaagctta ctggcttaac tatgcggcat cagagcagat
     7801 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7861 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7921 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7981 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8041 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8101 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8161 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8221 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8281 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8341 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8401 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8461 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8521 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8581 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8641 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8701 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8761 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8821 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8881 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8941 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9001 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9061 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9121 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9181 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9241 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9301 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9361 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9421 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9481 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9541 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9601 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9661 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9721 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9781 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     9841 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattagctt ggccattgca
     9901 tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc
     9961 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
    10021 tagcccatat atggagt
//
