LOCUS       sequence-102112-       12134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast dCas9 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12134)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 12134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC518
FEATURES             Location/Qualifiers
     source          1..12134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        2522..3188
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     3058..3081
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             join(3198..3221,3222..3224,3225..5711,5712..5714,
                     5715..7298)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7311..7331
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7335..7355
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7359..7379
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(8681..8700)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8682..8700)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8707..8724)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8707..8723)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8716..8738)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8864..9319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8951..8970)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9161..9182
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9675..9694)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9794..9816
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9854..9872)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9957..10061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10062..10130,10131..10922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10280..10299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11093..11681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11582..11601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11835..11852
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11969..11990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12005..12010,12011..12028,12029..12035)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12043..12059
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12048..12070
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12067..12083
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12067..12083
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        12104..12122
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccca gttcgagttt
     2521 atcattatca atactgccat ttcaaagaat acgtaaataa ttaatagtag tgattttcct
     2581 aactttattt agtcaaaaaa ttagcctttt aattctgctg taacccgtac atgcccaaaa
     2641 tagggggcgg gttacacaga atatataaca tcgtaggtgt ctgggtgaac agtttattcc
     2701 tggcatccac taaatataat ggagcccgct ttttaagctg gcatccagaa aaaaaaagaa
     2761 tcccagcacc aaaatattgt tttcttcacc aaccatcagt tcataggtcc attctcttag
     2821 cgcaactaca gagaacaggg gcacaaacag gcaaaaaacg ggcacaacct caatggagtg
     2881 atgcaacctg cctggagtaa atgatgacac aaggcaattg acccacgcat gtatctatct
     2941 cattttctta caccttctat taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa
     3001 aggttgaaac cagttccctg aaattattcc cctacttgac taataagtat ataaagacgg
     3061 taggtattga ttgtaattct gtaaatctat ttcttaaact tcttaaattc tacttttata
     3121 gttagtcttt tttttagttt taaaacacca agaacttagt ttcgaataaa cacacataaa
     3181 caaacaaaat gctcgaggac aagaagtatt ctatcggact ggccatcggg actaatagcg
     3241 tcgggtgggc cgtgatcact gacgagtaca aggtgccctc taagaagttc aaggtgctcg
     3301 ggaacaccga ccggcattcc atcaagaaaa atctgatcgg agctctcctc tttgattcag
     3361 gggagaccgc tgaagcaacc cgcctcaagc ggactgctag acggcggtac accaggagga
     3421 agaaccggat ttgttacctt caagagatat tctccaacga aatggcaaag gtcgacgaca
     3481 gcttcttcca taggctggaa gaatcattcc tcgtggaaga ggataagaag catgaacggc
     3541 atcccatctt cggtaatatc gtcgacgagg tggcctatca cgagaaatac ccaaccatct
     3601 accatcttcg caaaaagctg gtggactcaa ccgacaaggc agacctccgg cttatctacc
     3661 tggccctggc ccacatgatc aagttcagag gccacttcct gatcgagggc gacctcaatc
     3721 ctgacaatag cgatgtggat aaactgttca tccagctggt gcagacttac aaccagctct
     3781 ttgaagagaa ccccatcaat gcaagcggag tcgatgccaa ggccattctg tcagcccggc
     3841 tgtcaaagag ccgcagactt gagaatctta tcgctcagct gccgggtgaa aagaaaaatg
     3901 gactgttcgg gaacctgatt gctctttcac ttgggctgac tcccaatttc aagtctaatt
     3961 tcgacctggc agaggatgcc aagctgcaac tgtccaagga cacctatgat gacgatctcg
     4021 acaacctcct ggcccagatc ggtgaccaat acgccgacct tttccttgct gctaagaatc
     4081 tttctgacgc catcctgctg tctgacattc tccgcgtgaa cactgaaatc accaaggccc
     4141 ctctttcagc ttcaatgatt aagcggtatg atgagcacca ccaggacctg accctgctta
     4201 aggcactcgt ccggcagcag cttccggaga agtacaagga aatcttcttt gaccagtcaa
     4261 agaatggata cgccggctac atcgacggag gtgcctccca agaggaattt tataagttta
     4321 tcaaacctat ccttgagaag atggacggca ccgaagagct cctcgtgaaa ctgaatcggg
     4381 aggatctgct gcggaagcag cgcactttcg acaatgggag cattccccac cagatccatc
     4441 ttggggagct tcacgccatc cttcggcgcc aagaggactt ctaccccttt cttaaggaca
     4501 acagggagaa gattgagaaa attctcactt tccgcatccc ctactacgtg ggacccctcg
     4561 ccagaggaaa tagccggttt gcttggatga ccagaaagtc agaagaaact atcactccct
     4621 ggaacttcga agaggtggtg gacaagggag ccagcgctca gtcattcatc gaacggatga
     4681 ctaacttcga taagaacctc cccaatgaga aggtcctgcc gaaacattcc ctgctctacg
     4741 agtactttac cgtgtacaac gagctgacca aggtgaaata tgtcaccgaa gggatgagga
     4801 agcccgcatt cctgtcaggc gaacaaaaga aggcaattgt ggaccttctg ttcaagacca
     4861 atagaaaggt gaccgtgaag cagctgaagg aggactattt caagaaaatt gaatgcttcg
     4921 actctgtgga gattagcggg gtcgaagatc ggttcaacgc aagcctgggt acctaccatg
     4981 atctgcttaa gatcatcaag gacaaggatt ttctggacaa tgaggagaac gaggacatcc
     5041 ttgaggacat tgtcctgact ctcactctgt tcgaggaccg ggaaatgatc gaggagaggc
     5101 ttaagaccta cgcccatctg ttcgacgata aagtgatgaa gcaacttaaa cggagaagat
     5161 ataccggatg gggacgcctt agccgcaaac tcatcaacgg aatccgggac aaacagagcg
     5221 gaaagaccat tcttgatttc cttaagagcg acggattcgc taatcgcaac ttcatgcaac
     5281 ttatccatga tgattccctg acctttaagg aggacatcca gaaggcccaa gtgtctggac
     5341 aaggtgactc actgcacgag catatcgcaa atctggctgg ttcacccgct attaagaagg
     5401 gtattctcca gaccgtgaaa gtcgtggacg agctggtcaa ggtgatgggt cgccataaac
     5461 cagagaacat tgtcatcgag atggccaggg aaaaccagac tacccagaag ggacagaaga
     5521 acagcaggga gcggatgaaa agaattgagg aagggattaa ggagctcggg tcacagatcc
     5581 ttaaagagca cccggtggaa aacacccagc ttcagaatga gaagctctat ctgtactacc
     5641 ttcaaaatgg acgcgatatg tatgtggacc aagagcttga tatcaacagg ctctcagact
     5701 acgacgtgga cgccatcgtc cctcagagct tcctcaaaga cgactcaatt gacaataagg
     5761 tgctgactcg ctcagacaag aaccggggaa agtcagataa cgtgccctca gaggaagtcg
     5821 tgaaaaagat gaagaactat tggcgccagc ttctgaacgc aaagctgatc actcagcgga
     5881 agttcgacaa tctcactaag gctgagaggg gcggactgag cgaactggac aaagcaggat
     5941 tcattaaacg gcaacttgtg gagactcggc agattactaa acatgtcgcc caaatccttg
     6001 actcacgcat gaataccaag tacgacgaaa acgacaaact tatccgcgag gtgaaggtga
     6061 ttaccctgaa gtccaagctg gtcagcgatt tcagaaagga ctttcaattc tacaaagtgc
     6121 gggagatcaa taactatcat catgctcatg acgcatatct gaatgccgtg gtgggaaccg
     6181 ccctgatcaa gaagtaccca aagctggaaa gcgagttcgt gtacggagac tacaaggtct
     6241 acgacgtgcg caagatgatt gccaaatctg agcaggagat cggaaaggcc accgcaaagt
     6301 acttcttcta cagcaacatc atgaatttct tcaagaccga aatcaccctt gcaaacggtg
     6361 agatccggaa gaggccgctc atcgagacta atggggagac tggcgaaatc gtgtgggaca
     6421 agggcagaga tttcgctacc gtgcgcaaag tgctttctat gcctcaagtg aacatcgtga
     6481 agaaaaccga ggtgcaaacc ggaggctttt ctaaggaatc aatcctcccc aagcgcaact
     6541 ccgacaagct cattgcaagg aagaaggatt gggaccctaa gaagtacggc ggattcgatt
     6601 caccaactgt ggcttattct gtcctggtcg tggctaaggt ggaaaaagga aagtctaaga
     6661 agctcaagag cgtgaaggaa ctgctgggta tcaccattat ggagcgcagc tccttcgaga
     6721 agaacccaat tgactttctc gaagccaaag gttacaagga agtcaagaag gaccttatca
     6781 tcaagctccc aaagtatagc ctgttcgaac tggagaatgg gcggaagcgg atgctcgcct
     6841 ccgctggcga acttcagaag ggtaatgagc tggctctccc ctccaagtac gtgaatttcc
     6901 tctaccttgc aagccattac gagaagctga aggggagccc cgaggacaac gagcaaaagc
     6961 aactgtttgt ggagcagcat aagcattatc tggacgagat cattgagcag atttccgagt
     7021 tttctaaacg cgtcattctc gctgatgcca acctcgataa agtccttagc gcatacaata
     7081 agcacagaga caaaccaatt cgggagcagg ctgagaatat catccacctg ttcaccctca
     7141 ccaatcttgg tgcccctgcc gcattcaagt acttcgacac caccatcgac cggaaacgct
     7201 atacctccac caaagaagtg ctggacgcca ccctcatcca ccagagcatc accggacttt
     7261 acgaaactcg gattgacctc tcacagctcg gaggggatga gggagctgat ccaaaaaaga
     7321 agagaaaggt agatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtat
     7381 agaattctac cggcggccgc caccgcggtg gagctctaag caaatagcta aattatatac
     7441 gaattaatat tatgattaag tgtttacgtg agtgcgatat ttttattact atcttataca
     7501 gttgtatata ctctataaaa tgagttgtct attaattaac gcgatgaatg ctttctgggt
     7561 ttacctctcc aacaactcta gtttacttct caatacattc aattgtattt gatttgtcaa
     7621 tacttcatca ttaatcaatt ctatagtttt gtttttctcg tttatttcca aatttaatgc
     7681 atcaatttta ttattcaatt tgtcgttgat tttggttaat gattttatgg tttgatctct
     7741 ggcattgatt gtttgtgtta gtttttcatt attgataatt aaattattta agttagttat
     7801 caactcggtg ttttcaagtt tcaagttttc aatttcttta gagtttatta gatttgtcaa
     7861 agtttctgaa ttgcttgatt ggtcctgtag aagagtattt gttgttgtgg ataattgatt
     7921 caatttttga gacaattgct ggaaggcgtt gaaatatcta gcatcaatct catggttttt
     7981 ttcccgagag tctcgtagat tcaattgttt taatatatct tgggaccact cttgatttga
     8041 actcatggaa attaaactgg gtgttgtgtt gtggtgtaat gattgtaccc cctttgctta
     8101 taattgtgtg gccaccgaag tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc
     8161 ttaaaaagat tctctttttt tatgatattt gtacataaac tttataaatg aaattcataa
     8221 tagaaacgac acgaaattac aaaatggaat atgttcatag ggtagacgaa actatatacg
     8281 caatctacat acatttatca agaaggagaa aaaggaggat gtaaaggaat acaggtaagc
     8341 aaattgatac taatggctca acgtgataag gaaaaagaat tgcactttaa cattaatatt
     8401 gacaaggagg agggcaccac acaaaaagtt aggtgtaaca gaaaatcatg aaactatgat
     8461 tcctaattta tatattggag gattttctct aaaaaaaaaa aaatacaaca aataaaaaac
     8521 actcaatgac ctgaccattt gatggagttt aagtcaatac cttcttgaac catttcccat
     8581 aatggtgaaa gttccctcaa gaattttact ctgtcagaaa cggccttaac gacgtaggcc
     8641 ggccatttaa atgtttaaac ggccacgctg gcccaattcg ccctatagtg agtcgtatta
     8701 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     8761 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8821 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     8881 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8941 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9001 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9061 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9121 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9181 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9241 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     9301 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     9361 cacaccgcat agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt
     9421 attaggatct ctacccttgg cgaaaagtcc tctgccaaca atgatgatat ctgatccacc
     9481 acttacaact tcgtcgacgg ttctgtactg ctgacccaat atgcgtatat ataccaatct
     9541 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
     9601 tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaaggtggta
     9661 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     9721 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     9781 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     9841 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     9901 gtttcttaga cgtcggcgcg ccgaggacgt caggtggcac ttttcgggga aatgtgcgcg
     9961 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10021 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10081 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10141 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10201 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10261 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10321 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10381 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10441 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10501 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10561 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10621 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    10681 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    10741 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10801 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10861 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10921 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10981 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11041 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11101 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11161 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11221 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11281 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11341 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11401 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11461 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    11521 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    11581 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    11641 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    11701 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    11761 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    11821 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    11881 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    11941 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12001 aggctttaca ctttatgctt ccggctccta tgttgtgtgg aattgtgagc ggataacaat
    12061 ttcacacagg aaacagctat gaccatgatt acgccaagct cggaattaac cctcactaaa
    12121 gggaacaaaa gctg
//
