LOCUS       sequence-102117-       14615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MCP-VP64 and dCas9 in Yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 14615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC638
FEATURES             Location/Qualifiers
     source          1..14615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        3292..3996
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4024..4044
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4417..4566
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        4991..5655
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     primer_bind     complement(5004..5027)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    5322..5439
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             join(5679..5702,5703..5705,5706..8192,8193..8195,
                     8196..9779)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9792..9812
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9816..9836
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9840..9860
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(11162..11181)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11163..11181)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11188..11205)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11188..11204)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11197..11219)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11345..11800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11432..11451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11642..11663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12156..12175)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12275..12297
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12335..12353)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12438..12542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12543..12611,12612..13403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12761..12780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13574..14162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14063..14082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14316..14333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14450..14471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14486..14491,14492..14509,14510..14516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14524..14540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14529..14551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14548..14564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14548..14564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        14585..14603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccta aaacaagaag
     2521 agggttgact acatcacgat gagggggatc gaagaaatga tggtaaatga aataggaaat
     2581 caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg
     2641 ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc
     2701 atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct gggcgtgagg
     2761 ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc gctaaggggc
     2821 gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac cacgacaact
     2881 ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga gtatactaga
     2941 agaatgagcc aagacttgcg agacgcgagt ttgccggtgg tgcgaacaat agagcgacca
     3001 tgaccttgaa ggtgagacgc gcataaccgc tagagtactt tgaagaggaa acagcaatag
     3061 ggttgctacc agtataaata gacaggtaca tacaacactg gaaatggttg tctgtttgag
     3121 tacgctttca attcatttgg gtgtgcactt tattatgtta caatatggaa gggaacttta
     3181 cacttctcct atgcacatat attaattaaa gtccaatgct agtagagaag gggggtaaca
     3241 cccctccgcg ctcttttccg atttttttct aaaccgtgga atatttcgga tatccttttg
     3301 ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg
     3361 attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa
     3421 tagaacagat accagacagg acataatggg ctaaacaaga ctacaccaat tacactgcct
     3481 cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat
     3541 atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc
     3601 aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa
     3661 tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
     3721 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
     3781 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
     3841 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
     3901 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
     3961 acaatatttc aagctatacc aagcatacaa tcaactatct catatacaat gtctctcgag
     4021 atgcccaaaa agaaaagaaa agtgggtagt atggcttcta actttactca gttcgttctc
     4081 gtcgacaatg gcggaactgg cgacgtgact gtcgccccaa gcaacttcgc taacgggatc
     4141 gctgaatgga tcagctctaa ctcgcgttca caggcttaca aagtaacctg tagcgttcgt
     4201 cagagctctg cgcagaatcg caaatacacc atcaaagtcg aggtgcctaa aggcgcctgg
     4261 cgttcgtact taaatatgga actaaccatt ccaattttcg ccacgaattc cgactgcgag
     4321 cttattgtta aggcaatgca aggtctccta aaagatggaa acccgattcc ctcagcaatc
     4381 gcagcaaact ccggcatcta cggctccgga cgagccgatg ctctcgacga ttttgacctc
     4441 gatatgctgg gatccgacgc tctcgatgac ttcgaccttg acatgctggg gagtgacgct
     4501 ctggacgact tcgatctcga catgctgggc tccgacgccc tggatgactt cgatctggac
     4561 atgctcatca actgaagtgc ttttaactaa gaattattag tcttttctgc ttattttttc
     4621 atcatagttt agaacacttt atattaacga atagtttatg aatctattta ggtttaaaaa
     4681 ttgatacagt tttataagtt actttttcaa agactcgtgc tgtctattgc ataatgcact
     4741 ggaaggggaa aaaaaaggtg cacacgcgtg gctttttctt gaatttgcag tttgaaaaat
     4801 aactacatgg atgataagaa aacatggagt acagtcactt tgagaacctt caatcagctg
     4861 gtaacgtctt cgttaattgg atactcaaaa aagatggata gcatgaatca caagatggaa
     4921 ggaaatgcgg gccacgacca cagtgatatg catatgggag atggagatga tacctggccc
     4981 cggtgctata gttttttctc cttgacgtta aagtatagag gtatattaac aattttttgt
     5041 tgatactttt attacatttg aataagaagt aatacaaacc gaaaatgttg aaagtattag
     5101 ttaaagtggt tatgcagttt ttgcatttat atatctgtta atagatcaaa aatcatcgct
     5161 tcgctgatta attaccccag aaataaggct aaaaaactaa tcgcattatc atcctatggt
     5221 tgttaatttg attcgttcat ttgaaggttt gtggggccag gttactgcca atttttcctc
     5281 ttcataacca taaaagctag tattgtagaa tctttattgt tcggagcagt gcggcgcgag
     5341 gcacatctgc gtttcaggaa cgcgaccggt gaagacgagg acgcacggag gagagtcttc
     5401 cttcggaggg ctgtcacccg ctcggcggct tctaatccgt acttcaatat agcaatgagc
     5461 agttaagcgt attactgaaa gttccaaaga gaaggttttt ttaggctaag ataatggggc
     5521 tctttacatt tccacaacat ataagtaaga ttagatatgg atatgtatat ggatatgtat
     5581 atggtggtaa tgccatgtaa tatgattatt aaacttcttt gcgtccatcc aaaaaaaaag
     5641 taagaatttt tgaaaattca atataaatga tgctcgagga caagaagtat tctatcggac
     5701 tggccatcgg gactaatagc gtcgggtggg ccgtgatcac tgacgagtac aaggtgccct
     5761 ctaagaagtt caaggtgctc gggaacaccg accggcattc catcaagaaa aatctgatcg
     5821 gagctctcct ctttgattca ggggagaccg ctgaagcaac ccgcctcaag cggactgcta
     5881 gacggcggta caccaggagg aagaaccgga tttgttacct tcaagagata ttctccaacg
     5941 aaatggcaaa ggtcgacgac agcttcttcc ataggctgga agaatcattc ctcgtggaag
     6001 aggataagaa gcatgaacgg catcccatct tcggtaatat cgtcgacgag gtggcctatc
     6061 acgagaaata cccaaccatc taccatcttc gcaaaaagct ggtggactca accgacaagg
     6121 cagacctccg gcttatctac ctggccctgg cccacatgat caagttcaga ggccacttcc
     6181 tgatcgaggg cgacctcaat cctgacaata gcgatgtgga taaactgttc atccagctgg
     6241 tgcagactta caaccagctc tttgaagaga accccatcaa tgcaagcgga gtcgatgcca
     6301 aggccattct gtcagcccgg ctgtcaaaga gccgcagact tgagaatctt atcgctcagc
     6361 tgccgggtga aaagaaaaat ggactgttcg ggaacctgat tgctctttca cttgggctga
     6421 ctcccaattt caagtctaat ttcgacctgg cagaggatgc caagctgcaa ctgtccaagg
     6481 acacctatga tgacgatctc gacaacctcc tggcccagat cggtgaccaa tacgccgacc
     6541 ttttccttgc tgctaagaat ctttctgacg ccatcctgct gtctgacatt ctccgcgtga
     6601 acactgaaat caccaaggcc cctctttcag cttcaatgat taagcggtat gatgagcacc
     6661 accaggacct gaccctgctt aaggcactcg tccggcagca gcttccggag aagtacaagg
     6721 aaatcttctt tgaccagtca aagaatggat acgccggcta catcgacgga ggtgcctccc
     6781 aagaggaatt ttataagttt atcaaaccta tccttgagaa gatggacggc accgaagagc
     6841 tcctcgtgaa actgaatcgg gaggatctgc tgcggaagca gcgcactttc gacaatggga
     6901 gcattcccca ccagatccat cttggggagc ttcacgccat ccttcggcgc caagaggact
     6961 tctacccctt tcttaaggac aacagggaga agattgagaa aattctcact ttccgcatcc
     7021 cctactacgt gggacccctc gccagaggaa atagccggtt tgcttggatg accagaaagt
     7081 cagaagaaac tatcactccc tggaacttcg aagaggtggt ggacaaggga gccagcgctc
     7141 agtcattcat cgaacggatg actaacttcg ataagaacct ccccaatgag aaggtcctgc
     7201 cgaaacattc cctgctctac gagtacttta ccgtgtacaa cgagctgacc aaggtgaaat
     7261 atgtcaccga agggatgagg aagcccgcat tcctgtcagg cgaacaaaag aaggcaattg
     7321 tggaccttct gttcaagacc aatagaaagg tgaccgtgaa gcagctgaag gaggactatt
     7381 tcaagaaaat tgaatgcttc gactctgtgg agattagcgg ggtcgaagat cggttcaacg
     7441 caagcctggg tacctaccat gatctgctta agatcatcaa ggacaaggat tttctggaca
     7501 atgaggagaa cgaggacatc cttgaggaca ttgtcctgac tctcactctg ttcgaggacc
     7561 gggaaatgat cgaggagagg cttaagacct acgcccatct gttcgacgat aaagtgatga
     7621 agcaacttaa acggagaaga tataccggat ggggacgcct tagccgcaaa ctcatcaacg
     7681 gaatccggga caaacagagc ggaaagacca ttcttgattt ccttaagagc gacggattcg
     7741 ctaatcgcaa cttcatgcaa cttatccatg atgattccct gacctttaag gaggacatcc
     7801 agaaggccca agtgtctgga caaggtgact cactgcacga gcatatcgca aatctggctg
     7861 gttcacccgc tattaagaag ggtattctcc agaccgtgaa agtcgtggac gagctggtca
     7921 aggtgatggg tcgccataaa ccagagaaca ttgtcatcga gatggccagg gaaaaccaga
     7981 ctacccagaa gggacagaag aacagcaggg agcggatgaa aagaattgag gaagggatta
     8041 aggagctcgg gtcacagatc cttaaagagc acccggtgga aaacacccag cttcagaatg
     8101 agaagctcta tctgtactac cttcaaaatg gacgcgatat gtatgtggac caagagcttg
     8161 atatcaacag gctctcagac tacgacgtgg acgccatcgt ccctcagagc ttcctcaaag
     8221 acgactcaat tgacaataag gtgctgactc gctcagacaa gaaccgggga aagtcagata
     8281 acgtgccctc agaggaagtc gtgaaaaaga tgaagaacta ttggcgccag cttctgaacg
     8341 caaagctgat cactcagcgg aagttcgaca atctcactaa ggctgagagg ggcggactga
     8401 gcgaactgga caaagcagga ttcattaaac ggcaacttgt ggagactcgg cagattacta
     8461 aacatgtcgc ccaaatcctt gactcacgca tgaataccaa gtacgacgaa aacgacaaac
     8521 ttatccgcga ggtgaaggtg attaccctga agtccaagct ggtcagcgat ttcagaaagg
     8581 actttcaatt ctacaaagtg cgggagatca ataactatca tcatgctcat gacgcatatc
     8641 tgaatgccgt ggtgggaacc gccctgatca agaagtaccc aaagctggaa agcgagttcg
     8701 tgtacggaga ctacaaggtc tacgacgtgc gcaagatgat tgccaaatct gagcaggaga
     8761 tcggaaaggc caccgcaaag tacttcttct acagcaacat catgaatttc ttcaagaccg
     8821 aaatcaccct tgcaaacggt gagatccgga agaggccgct catcgagact aatggggaga
     8881 ctggcgaaat cgtgtgggac aagggcagag atttcgctac cgtgcgcaaa gtgctttcta
     8941 tgcctcaagt gaacatcgtg aagaaaaccg aggtgcaaac cggaggcttt tctaaggaat
     9001 caatcctccc caagcgcaac tccgacaagc tcattgcaag gaagaaggat tgggacccta
     9061 agaagtacgg cggattcgat tcaccaactg tggcttattc tgtcctggtc gtggctaagg
     9121 tggaaaaagg aaagtctaag aagctcaaga gcgtgaagga actgctgggt atcaccatta
     9181 tggagcgcag ctccttcgag aagaacccaa ttgactttct cgaagccaaa ggttacaagg
     9241 aagtcaagaa ggaccttatc atcaagctcc caaagtatag cctgttcgaa ctggagaatg
     9301 ggcggaagcg gatgctcgcc tccgctggcg aacttcagaa gggtaatgag ctggctctcc
     9361 cctccaagta cgtgaatttc ctctaccttg caagccatta cgagaagctg aaggggagcc
     9421 ccgaggacaa cgagcaaaag caactgtttg tggagcagca taagcattat ctggacgaga
     9481 tcattgagca gatttccgag ttttctaaac gcgtcattct cgctgatgcc aacctcgata
     9541 aagtccttag cgcatacaat aagcacagag acaaaccaat tcgggagcag gctgagaata
     9601 tcatccacct gttcaccctc accaatcttg gtgcccctgc cgcattcaag tacttcgaca
     9661 ccaccatcga ccggaaacgc tatacctcca ccaaagaagt gctggacgcc accctcatcc
     9721 accagagcat caccggactt tacgaaactc ggattgacct ctcacagctc ggaggggatg
     9781 agggagctga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtagatc
     9841 caaaaaagaa gagaaaggta tagaattcta ccggcggccg ccaccgcggt ggagctctaa
     9901 gcaaatagct aaattatata cgaattaata ttatgattaa gtgtttacgt gagtgcgata
     9961 tttttattac tatcttatac agttgtatat actctataaa atgagttgtc tattaattaa
    10021 cgcgatgaat gctttctggg tttacctctc caacaactct agtttacttc tcaatacatt
    10081 caattgtatt tgatttgtca atacttcatc attaatcaat tctatagttt tgtttttctc
    10141 gtttatttcc aaatttaatg catcaatttt attattcaat ttgtcgttga ttttggttaa
    10201 tgattttatg gtttgatctc tggcattgat tgtttgtgtt agtttttcat tattgataat
    10261 taaattattt aagttagtta tcaactcggt gttttcaagt ttcaagtttt caatttcttt
    10321 agagtttatt agatttgtca aagtttctga attgcttgat tggtcctgta gaagagtatt
    10381 tgttgttgtg gataattgat tcaatttttg agacaattgc tggaaggcgt tgaaatatct
    10441 agcatcaatc tcatggtttt tttcccgaga gtctcgtaga ttcaattgtt ttaatatatc
    10501 ttgggaccac tcttgatttg aactcatgga aattaaactg ggtgttgtgt tgtggtgtaa
    10561 tgattgtacc ccctttgctt ataattgtgt ggccaccgaa gtcggtgatg ctgtcgccga
    10621 agaagttaag aaaatccttg cttaaaaaga ttctcttttt ttatgatatt tgtacataaa
    10681 ctttataaat gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata
    10741 gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga
    10801 tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa
    10861 ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt taggtgtaac
    10921 agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc taaaaaaaaa
    10981 aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt taagtcaata
    11041 ccttcttgaa ccatttccca taatggtgaa agttccctca agaattttac tctgtcagaa
    11101 acggccttaa cgacgtaggc cggccattta aatgtttaaa cggccacgct ggcccaattc
    11161 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
    11221 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    11281 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    11341 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    11401 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    11461 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    11521 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    11581 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    11641 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    11701 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    11761 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
    11821 ttacgcatct gtgcggtatt tcacaccgca tagggtaata actgatataa ttaaattgaa
    11881 gctctaattt gtgagtttag tattaggatc tctacccttg gcgaaaagtc ctctgccaac
    11941 aatgatgata tctgatccac cacttacaac ttcgtcgacg gttctgtact gctgacccaa
    12001 tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga
    12061 gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa
    12121 tgatgaattg aaaaggtggt atggtgcact ctcagtacaa tctgctctga tgccgcatag
    12181 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
    12241 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
    12301 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
    12361 gttaatgtca tgataataat ggtttcttag acgtcggcgc gccgaggacg tcaggtggca
    12421 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    12481 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    12541 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    12601 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    12661 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    12721 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    12781 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    12841 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    12901 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    12961 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    13021 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    13081 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    13141 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    13201 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    13261 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    13321 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    13381 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    13441 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    13501 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    13561 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    13621 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    13681 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    13741 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    13801 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    13861 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    13921 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    13981 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    14041 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    14101 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    14161 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    14221 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    14281 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    14341 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    14401 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    14461 agctcactca ttaggcaccc caggctttac actttatgct tccggctcct atgttgtgtg
    14521 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    14581 tcggaattaa ccctcactaa agggaacaaa agctg
//
