LOCUS       sequence-102129-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused to LINuS, a light-inducible nuclear localization 
            signal (biNLS10/PKIt NES).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di 
            Ventura B
  TITLE     Engineering light-inducible nuclear localization signals for precise
            spatiotemporal control of protein dynamics in living cells.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.
  PUBMED    25019686
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4404. doi"; pages: " 
            10.1038/ncomms5404"
COMMENT     Sequence Label: pDN42
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..86
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(978..997)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
       61 ctggcaacta gaaggcacag tcgaggctga tcagcggttt aaacttaagc ttggtaccga
      121 gctcggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg
      181 ctcgagctag tccagctttt tcttctttgc tgcggctgcg gctgctgcgg ccaggttggc
      241 gtcgggcagt ctctttgcgg cctcgtcaat attttctgca gttttcttaa tcagcatgac
      301 tccctctctc tcggcagcat ctcggacatg ctcagttcca tccaactgaa ccccaataaa
      361 gtactggaca tctcccttct gatctcgcat aggctgcaag tgaaagaggt tccagaactt
      421 tttaccactc tttgtataat taatcagctg aacagtgacc tctgtttggt tatctatggc
      481 atctctaatt tttctcactg tcgcgcgatc agtttcagga ccttgtagaa acctgcagtt
      541 tcttcccaaa atttcttcac ggctatattc tgtcaactgc aagaaactat cggacgcgaa
      601 tataatggga ttatctggca atcttgggtc agtaatgaca aagttcttct caatacgttc
      661 aagtgtagta gccaaacttc cacctgaacc tccagatcca cccttgtaca gctcgtccat
      721 gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc
      781 gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca gctgcacggg
      841 cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt ccttcagctt
      901 cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga
      961 ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc
     1021 gcgcagcttc accttgtaga tgaactcgcc gtcctg
//
