LOCUS       sequence-102134-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mCherry fused to LINuS, a light-inducible 
            nuclear localization signal (cMycP1A NLS/PKIt NES).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di 
            Ventura B
  TITLE     Engineering light-inducible nuclear localization signals for precise
            spatiotemporal control of protein dynamics in living cells.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.
  PUBMED    25019686
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4404. doi"; pages: " 
            10.1038/ncomms5404"
COMMENT     Sequence Label: pDB22
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..86
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(927..946)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
       61 ctggcaacta gaaggcacag tcgaggctga tcagcggttt aaacttaagc ttggtaccga
      121 gctcggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg
      181 ctcgagctag tccaacttga ccctcttggc agcggcaata ttttctgcag ttttcttaat
      241 cagcatgact ccctctctct cggcagcatc tcggacatgc tcagttccat ccaactgaac
      301 cccaataaag tactggacat ctcccttctg atctcgcata ggctgcaagt gaaagaggtt
      361 ccagaacttt ttaccactct ttgtataatt aatcagctga acagtgacct ctgtttggtt
      421 atctatggca tctctaattt ttctcactgt cgcgcgatca gtttcaggac cttgtagaaa
      481 cctgcagttt cttcccaaaa tttcttcacg gctatattct gtcaactgca agaaactatc
      541 ggacgcgaat ataatgggat tatctggcaa tcttgggtca gtaatgacaa agttcttctc
      601 aatacgttca agtgtagtag ccaaacttcc acctgaacct ccagatccac ccttgtacag
      661 ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt
      721 gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag
      781 ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc
      841 cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg
      901 ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa
      961 gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg
     1021 ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc
     1081 ggg
//
