LOCUS       sequence-102148-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-thyA::RBS B repA,
            Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support
            pSEVAT7Z..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12TEC-B
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..722)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ttaattaagc accggtggag
       61 tgacgacctt cagcacgttc gtactgttca acgatggtgt agtcttcgtt gtgggaggtg
      121 atgtccagtt tgatgtcggt tttgtaagca cccggcagct gaaccggttt tttagccatg
      181 taggtggttt taacttcagc gtcgtagtga ccaccgtctt tcagtttcag acgcattttg
      241 atttcacctt tcagagcacc gtcttccggg tacatacgtt cggtggaagc ttcccaaccc
      301 atggtttttt tctgcataac cggaccgtcg gacgggaagt tggtaccacg cagtttaact
      361 ttgtagatga actcaccgtc ttgcagggag gagtcctggg taacggtaac aacaccaccg
      421 tcttcgaagt tcataacacg ttcccatttg aaaccttccg ggaaggacag tttcaggtag
      481 tccgggatgt cagccgggtg tttaacgtaa gctttggaac cgtactggaa ctgcggggac
      541 aggatgtccc aagcgaacgg cagcggacca cctttggtaa ctttcagttt agcggtctgg
      601 gtaccttcgt acggacgacc ttcaccttca ccttcgattt cgaactcgtg accgttaacg
      661 gaaccttcca tacgaacttt gaaacgcatg aactctttga taacgtcttc ggaggaagcc
      721 atctagtatt caccaccttt ctctagtaac atttattgta caacacgagc ccatttttgt
      781 caaataaaat ttaaattata tcaacgttaa taagacgttg tcaataaaat tattttgaca
      841 aaattcctag gtctctgatt ttatcgcacc actcattccg tttggtcttg gtgccgggcg
      901 tctgggcaac tttattaacg gtgaattgtg gggccgcgtt gacccgaact tcccgtttgc
      961 catgctgttc cc
//
