LOCUS       sequence-102149-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-thyA::RBS B repA,
            Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support
            pSEVAT7Z..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12TEC-B
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttaattttcg gtagtacgtc gctcgactga tgcctaatgc ctcccacggt ttcagcgaac
       61 gtgctgatgt aggaactgct gcgcgcttag atttagttcc gcccttgcga ccgcgagcgg
      121 cctgtacagc ggaaaatccc tctggtgaga atttcctgtg tgtatatttc gcaatgctct
      181 tgccaatagc ccgacattca gccggagaaa gcggaacggg aagcgaagcg ttgtacattt
      241 cgacacgctg gataacggca tcaagccatt gtgagaatac aggccagccc tgacgaatcg
      301 ctctgtaggc ccatttacgg accttttcga acagatggta gtttcgcccc agcccgtaat
      361 ttttatcgac gctacggcgc gctgaggcgc tcagatccag ataatcagcc agttcgtcga
      421 gagtgtaggg ttcctcgcgc cattccactt cctgccattc agggtggcac ggatttttgc
      481 agatcaggcc gctgtaattc acatccgcgc ccagtttttc acacaacgca cgctcaatag
      541 cggcagcgta tctgagcgcc gaagccgatg catccggcgc agttctcaca ggaagggcga
      601 gcgcgtagag cagatgcgcg tgcccgttac ggggattttt tacggtgatg ttcggggccg
      661 gacaattccg gtcgctccag tcgattgatg cccccgtccg gtccacgtca aagggaagcc
      721 agtacagtgc atgtggctgg ttctgctgga tgaaacgggc aagaatggca atgtttttcg
      781 gcagaatgcg aacgccgtac gcgaaatcgt tagtacagta cggtttgtgc ggtaattttt
      841 cacggaagcg ttgaagtacg gcgctcacgt tcacttctca cagggctcgt tactgacg
//
