LOCUS       sequence-102151-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-thyA::RBS I repA,
            Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will 
            support pSEVAT7Z..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12TEC-I
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(385..418)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc
       61 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca
      121 accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc
      181 cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg
      241 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc
      301 ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg
      361 cagcgtgagc ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcga
      421 actgcaggtc gactcaagcc actttctctt tattcaaaac ttgttttgag taacgattca
      481 ttgaccgcca atactcatga acggcttgta attcttctaa agaataatca aaaagattta
      541 cacgttttgc aagctttaat tggtttaata aattgacttc caataattga gagtcatttt
      601 tatttaattc atggttcaaa acatagttta atactcgatt ataaacgctt gccgataatt
      661 cgttaatgat ctctatttca aatgtttttt catacgtaac tctgagagat cccctcataa
      721 tttccccaaa acgtaaccat gtgtgaatag attttgagta agcagggttg cagccacgag
      781 tgagtcttcc cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac
      841 tgttgagagt acgtttcgat ttctgactgt gttagcctg
//
