LOCUS       sequence-102154-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-thyA::RBS I repA,
            Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will 
            support pSEVAT7Z..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12TEC-I
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        690..724
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 acgaactttg aaacgcatga actctttgat aacgtcttcg gaggaagcca tctagtattc
       61 accacctttc tctagtaaca tttattgtac aacacgagcc catttttgtc aaataaaatt
      121 taaattatat caacgttaat aagacgttgt caataaaatt attttgacaa aattcctagg
      181 tctctgattt tatcgcacca ctcattccgt ttggtcttgg tgccgggcgt ctgggcaact
      241 ttattaacgg tgaattgtgg ggccgcgttg acccgaactt cccgtttgcc atgctgttcc
      301 ctggctcccg tacagaagat attttgctgc tgcaaaccaa cccgcagtgg caatccattt
      361 tcgacactta cggtgtgctg ccgcgccacc catcacagct ttacgagctg ctgctggaag
      421 gtgtggtgct gtttattatc ctcaacctgt atattcgtaa accacgccca atgggagctg
      481 tctcaggttt gttcctgatt ggttacggcg cgtttcgcat cattgttgag tttttccgcc
      541 agcccgacgc gcagtttacc ggtgcctggg tgcagtacat cagcatgggg caaattcttt
      601 ccatcccgat gattgtcgcg ggtgtgatca tgatggtctg ggcatatcgt cgcagcccac
      661 agcaacacgt ttcctgagga accgaattct tgacagctag ctcagtccta ggtataatgc
      721 tagcggatct cgtcagtaac gagccctggg agaaagatgg gtgagcgccg tacttcaacg
      781 cttccgtgaa aaattaccgc acaaaccgta ctgtactaac gatttcgcgt acggcgttcg
      841 cattctgccg aaaaacattg ccattcttgc ccgtttcatc cagcagaacc agccacatgc
      901 actgtactg
//
