LOCUS       sequence-102156-         529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible entry vector for direct cloning of artificial 
            microRNAs into rice MIR390 precursor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pENTR-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(88..94,95..112,113..118))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(203..302)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(318..335)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(318..334)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(327..349)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      398..484
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
       61 tcgacggctc ctaactcaaa atccacacat tatacgagcc ggaagcataa agtgtaaagc
      121 ctggggtgcc taatggtctc tcaaggattg ttccataccc ttcctcaaaa catctcgagc
      181 tcggtgaagg gggcggccgc ggagcctgct tttttgtaca aagttggcat tataaaaaag
      241 cattgctcat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
      301 tggggcccga gcttaagact ggccgtcgtt ttacaacgtc gtgactggga aaacatccat
      361 gctagcgtta acgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
      421 ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
      481 aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg cccggaggg
//
