LOCUS       sequence-102159-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into rice MIR390 precursor. To use for expressing amiRNAs 
            in monocots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pMDC32B-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    32..206
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt
       61 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt
      121 ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga
      181 actactcaca cattattatg gagaaactcg agcttgtcga tcgacagatc ccggtcggca
      241 tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
      301 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
      361 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
      421 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
      481 cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
      541 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
      601 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
      661 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg ac
//
