LOCUS       sequence-10216-e        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Guang W, Kim KC, Lillehoj EP
  TITLE     MUC1 mucin interacts with calcium-modulating cyclophilin ligand.
  JOURNAL   Int J Biochem Cell Biol. 2009 Jun . 41(6):1354-60.
  PUBMED    19135167
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J 
            Biochem Cell Biol. 2009 Jun . 41(6):1354-60."
COMMENT     Sequence Label: pcDNA-CAML-Myc-His
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             927..956
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             972..989
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctcgagccac catggagtcg atggccgtcg ctaccgacgg cggggagagg ccgggggtcc
       61 cagcgggctc aggtctgtcg gcttcccagc gtcgggcgga gctgcgtcgg agaaagctgc
      121 tcatgaactc ggaacagcgc atcaaccgga tcatgggctt tcacaggccc gggagcggcg
      181 cggaagaaga aagtcaaaca aaatcaaagc agcaggacag tgataaactg aactccctca
      241 gcgttccttc cgtttcaaag cgagtagtgc tgggtgattc agtcagtaca ggaacaactg
      301 accagcaggg tggtgtggcc gaggtaaagg ggacccaact gggagacaaa ttggactcgt
      361 tcattaaacc acctgagtgc agtagtgatg tcaaccttga gctccggcag cggaacagag
      421 gggacctgac agcggactcg gtccagaggg gttcccgcca tggcctagag cagtaccttt
      481 ccagattcga agaagcaatg aagctaagga aacagctgat tagtgaaaaa cccagtcaag
      541 aggatggaaa tacaacagaa gaatttgact cttttcgaat atttagattg gtgggatgtg
      601 ctcttcttgc tcttggagtc agagcttttg tttgcaaata cttgtccata tttgctccat
      661 ttcttacttt acaacttgcg tacatgggat tatacaaata ttttcccaag agtgaaaaga
      721 agataaagac aacagtacta acagctgcac ttctattgtc gggaattcct gccgaagtga
      781 taaatcgatc aatggatacc tatagcaaaa tgggcgaagt cttcacagat ctctgtgtct
      841 actttttcac ttttatcttt tgtcatgaac tgcttgatta ttggggctct gaagtaccgg
      901 atccgagctc ggtaccaagc tttctagaac aaaaactcat ctcagaagag gatctgaata
      961 gcgccgtcga ccatcatcat catcatcatt gagttaaacg tctccagcta
//
