LOCUS       sequence-102161-         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into rice MIR390 precursor. To use for expressing amiRNAs 
            in monocots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pMDC32B-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(71..77,78..95,96..101))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(186..210)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(270..614)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(306..326)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg ctcctaactc
       61 aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt gcctaatggt
      121 ctctcaagga ttgttccata cccttcctca aaacatctcg agctcggtga agggggcggc
      181 cgcggagcct gcttttttgt acaaacttgt ttgatagctt ggcgcgcctc gagggggggc
      241 ccggtacccg gggatcctct agagtcgagg tcctctccaa atgaaatgaa cttccttata
      301 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
      361 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
      421 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tcaacgatgg
      481 cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttcc actatcttca
      541 caataaagtg acagatagct gggcaatgga atccgaggag gtttccggat attacccttt
      601 gttgaaaagt ctcaattgcc ctttggtctt ctgagactg
//
