LOCUS       sequence-102169-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of artificial 
            microRNAs into rice MIR390 precursor. To use for expressing amiRNAs 
            in monocots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pMDC123SB-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(147..171)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      235..487
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(504..520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(504..520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(517..539)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    528..544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(552..558,559..576,577..582))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    597..618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
       61 acacagccag tctgcacctc gacggtctca catggtttgt tcttaccaca cgaccaatta
      121 aatcgagctc aagggtgggc gcgccgaccc agctttcttg tacaaagtgg ttcgataatt
      181 cttaattaac tagttctaga gcggccgcca ccgcggtgga gctcgaattt ccccgatcgt
      241 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
      301 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
      361 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
      421 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
      481 ctagatcggg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
      541 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      601 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      661 tcgtgccagc tgcattaatg
//
