LOCUS       sequence-102173-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pCaSpeR-act5cB-LexAQF
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(37..288,289..642)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
ORIGIN
        1 atgccaccca agaagaagcg aaaagtagaa gatccaatga aggctctcac ggcccgacaa
       61 caggaagttt ttgatttgat acgggatcat atatcccaaa cgggtatgcc tccgacccgc
      121 gcagagatag cacagcgact gggctttcga tcgcctaacg ccgcggagga gcacttgaag
      181 gcactggccc gcaagggtgt cattgaaatc gtgtccggtg cgagccgcgg aatccggctg
      241 ttgcaggagg aggaagaggg cctgccactg gtgggacgcg tggccgctgg cgagccgctg
      301 ctggcccagc aacacataga gggacactat caggtggacc cctccttgtt taagccaaat
      361 gctgatttcc tgttgcgggt gtcgggaatg tccatgaagg acatcggtat tatggatggt
      421 gacctcctcg ccgtccataa gacacaggat gtcaggaacg gccaggtagt cgttgccagg
      481 atagacgatg aggtcactgt gaaacgtctc aagaagcaag gcaataaggt cgagctgctg
      541 ccggagaata gcgagttcaa gccgatcgtg gtggatctgc gacagcagtc ctttactatc
      601 gagggcttgg ccgtgggtgt gatccgcaac ggagattggc tgggacgtca gttggagctc
      661 cctcctaccg ccacggctac ggcctcgata atgccgcacg tgatggagca gcctctcagt
      721 accagcatta accccgtcaa cgaccgcttc aacggtattc c
//
