LOCUS       sequence-102175-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pCaSpeR-act5cB-LexAQFw
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..28
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(38..289,290..643)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
ORIGIN
        1 aatgccaccc aagaagaagc gaaaagtaga agatccaatg aaggctctca cggcccgaca
       61 acaggaagtt tttgatttga tacgggatca tatatcccaa acgggtatgc ctccgacccg
      121 cgcagagata gcacagcgac tgggctttcg atcgcctaac gccgcggagg agcacttgaa
      181 ggcactggcc cgcaagggtg tcattgaaat cgtgtccggt gcgagccgcg gaatccggct
      241 gttgcaggag gaggaagagg gcctgccact ggtgggacgc gtggccgctg gcgagccgct
      301 gctggcccag caacacatag agggacacta tcaggtggac ccctccttgt ttaagccaaa
      361 tgctgatttc ctgttgcggg tgtcgggaat gtccatgaag gacatcggta ttatggatgg
      421 tgacctcctc gccgtccata agacacagga tgtcaggaac ggccaggtag tcgttgccag
      481 gatagacgat gaggtcactg tgaaacgtct caagaagcaa ggcaataagg tcgagctgct
      541 gccggagaat agcgagttca agccgatcgt ggtggatctg cgacagcagt cctttactat
      601 cgagggcttg gccgtgggtg tgatccgcaa cggagattgg ctgggacgtc agttggagct
      661 ccctcctacc gccacggcta cggcctcgat aatgccgcac gtgatggagc agcctctcag
      721 ta
//
