LOCUS       sequence-102202-        7138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre recombinase-expressing helper plasmid for Anopheles gene 
            engineering, vasa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pENTR L1-vas2-Cre-L4
FEATURES             Location/Qualifiers
     source          1..7138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3058..3078
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3097..3123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3178..3198
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3217..4248
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     protein_bind    complement(5253..5348)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5379..5398)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5380..5398)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5403..5419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5403..5419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5521..5540)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5532..6341
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5599..5618)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6488..7076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6977..6996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgg taccgggccc cccgctagcg tcgacggtat cgatgtagaa cgcgagcaaa
      721 ttcttttcct tccatgacag cagcagctac agtgggaagc cgaacgtcag acgtgtttga
      781 catgccgaac tgggcgggaa aattacagcg tgcgctttgt tttcaagcaa atcacaactc
      841 gctgcaaaca aaaccgttga gaaattgatt gttttataat ttgtattgta ttttatttgt
      901 tataataaac taaaaagaca tactttttgc atattttata cataaaaaca tacatgcagc
      961 attataaaac acatataaac cctccctgta gagtcccgta tcgaaatctt ccatcctagt
     1021 tgcacagtac gacggacgag taggccgtgt ccgtgcaaat tccagctttt agcagtcttt
     1081 tgctcggagc actcgcggcg agtcggaggt ttctgctgag gtgcttagcg ctaaattagc
     1141 caattgcttt tgcaagtgaa ataaccagcc gaatagtact tcaaaactca ggtaagtgaa
     1201 ctagttttat agaacaaatg tttgtttgtt agaagttagt gaagtgtttg tgaaaaaaat
     1261 ctctcatttc ggcaaaacta acgtaactga tttcaaattg aattattgtt ttgtgatgtt
     1321 atattatttc atccagttga ttagtatttt cttagttatg ttcaaaatac agttaaatta
     1381 aatttcattt catttactca taaaataatc tcttggctta tttaattttt ctcgaattcg
     1441 cttgtattgt tcagtagcac gcgccattcg ccctttgttt cattttgtac ctgctcccac
     1501 taacacactg gcagtgcgaa acaaaagcct tcgcacgcgt tgctggtatt agagtgtgtg
     1561 cgtgtgtgtg ttgagcgctc tgtcaaaatc ggctgttgcc gccggtaccg aaattgcctg
     1621 ttcgcacgct gttcgtaaac attccgtggt gtgtatcgtg tgttgtgcat gttgcgcgcc
     1681 tccccccttt tgatagcagg ctgccgtggc tgccgtggtg tgtggcgcag ttgagttttt
     1741 ggattaattt tctaaggaaa tggcacgaga agagcggtgg cagtgtgttg gtttgctctg
     1801 tcccttcctt tctgtgtgaa gtgttcttac agcacagcac gtatccacca ccgcacacag
     1861 agcaggcaag gaagtggaag tgaacaagtg tgctgcgcat gcatgtgtgt ggggggcatt
     1921 ttagctgaga tcgtcgttat ttgagaagcg gtataggggc cagtcggtgt cgacgtacgg
     1981 aagcggttta gttttaatcc aagcgtatcc cgtcgtggag tggttgtgtg gctctgtgtg
     2041 ctctcatatc agttccagag tgaggttagt agaatcacag tccttggcct ttttcgttac
     2101 aagatatcca gaaggatggc gttatttcca cagcttacca tggtgctctt gtttgctcga
     2161 atcaggggag aaaaacagtt tcgtgtttca tgaaccgcag ttggcactgg agcggattca
     2221 aaagtcttcg atatgcaata gataagagag tcgttggggc atagttggga agcctttccg
     2281 agatgtggag tttccgagag gagaaatggt gctttcgtgc acgttccggg acagcgggcc
     2341 ccgcgaagag catctcgttg tcgttcatcc ggcaataatt gatgcgaaaa gcgcgcgcgc
     2401 cactggctta gcgcagtgta cacagtgata ttcacctaca cacacagagg cacacgcctt
     2461 cacacgcgcg cgtgcttcaa aggctacttc ggtggcggtg tgtgaggtcg cttgcaatgg
     2521 acaatgaaaa tttcgctgga aaataccatc gtctctttag gttgcaatgg gtgcgggtag
     2581 agcggtggtc gtcgatattg gtggtgtagt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     2641 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     2701 gtgcaacggc aattattttt tgtaatattt cgaccatctt tctttctctc tctccacgtg
     2761 ctgctgctgt tgctgctgct gctgcattgc atgttccact attcctctcg gtttgtgcct
     2821 gcggacgcca ttgctagtcg aaagagagtc gccgttagtc gcgcttcgag caacggacac
     2881 gttttttggt tgaaaccaac agcttttttc atcttcggga gacacacaga tctcgaatcg
     2941 tacattccca taaggagaat tgtcatcttc cggtgaataa agaaaggaaa cggatccgga
     3001 aacatgggat catcatcaga cgacgaagct acggccgatg cgcagcatgc agcaccacca
     3061 aaaaaaaaac gaaaagtaga agacccacga tttatgtacc catacgatgt tcctgactat
     3121 gcgggtatga aaaacatcaa aaaaaaccag ggaattcgcc ctttcatgag cggccctcca
     3181 aaaaagaaga gaaaggtaga agacccgggc ggccgcatgt ccaatttact gaccgtacac
     3241 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
     3301 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
     3361 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
     3421 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc
     3481 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
     3541 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
     3601 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
     3661 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
     3721 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
     3781 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
     3841 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
     3901 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     3961 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     4021 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     4081 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     4141 atcatgcaag ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg
     4201 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattagcc atgtacctcg
     4261 agcttggggt ggggttgtta tgtgttgcga acgagagtgg atctctctcg acatttcctt
     4321 attttttttt tctattgtta acacttacaa cgaaacttcg gaagagaagt ttcctcattc
     4381 gaaacgagga gtcaaactcc tttccttgct tttgtgacat gcatgattat tctttcattt
     4441 actgacgtaa cgatgtaaaa cacacagaag aagcatgaca cacagcaaag aattgttcgt
     4501 taataaaacg ttgatgaaaa ctttgaaaac ataagaactt gcacttttat tctataattc
     4561 gtgaaagctt gcaccgattg tctttcatta ttcaatgtaa tgtactgaaa ggtgattttt
     4621 cgcacttgta tactctagaa ctgaagtatt ctaacaatac gtcaccttta ggtccattcc
     4681 aggataaaat acacaagtga aggtagttgt acaaagtact tagactaacc caggtttcaa
     4741 aaaagataac gacgaattga gcgtaactgc acaaagcccg ttcattgtca acatatcgtt
     4801 caggggtctt caaactttat tatcagcccg cgggccgctt agagcaccat cttgatgtat
     4861 cctttatcta gtggcttatc gtttatgtct cgtttggcta agaaaatact aataacatat
     4921 tcaatttacg gagcggtacc atggtacaaa cgtcatactg ctagacttga caacatgccc
     4981 atcgagggtt cttacctcaa atggaccgat cccttgtaac tggcggcgta aactcagtaa
     5041 attccaaaag ccagtatagg ccaccatgac cgtctaacta ttccaaaaag gagaatattt
     5101 gaaatttcta tatctatagt attattacta tatctatatg tttctttagt acaatattag
     5161 agcttgatta ttttcctgaa aattgtaaaa tatgcatgtt agatggttga tacatcgttt
     5221 taaaaaaaac tgctgttaat ggctcgagca cccaactttt ctatacaaag ttggcattat
     5281 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     5341 cattatttgc catccagctg cagggcggcc gcgatatccc ctatagtgag tcgtattaca
     5401 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     5461 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     5521 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     5581 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     5641 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     5701 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     5761 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     5821 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     5881 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     5941 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     6001 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     6061 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     6121 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     6181 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     6241 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     6301 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     6361 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     6421 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6601 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6841 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6901 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
