LOCUS       sequence-102223-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mir124a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Cassady JP, D'Alessio AC, Sarkar S, Dani VS, Fan ZP, Ganz K, 
            Roessler R, Sur M, Young RA, Jaenisch R
  TITLE     Direct Lineage Conversion of Adult Mouse Liver Cells and B 
            Lymphocytes to Neural Stem Cells.
  JOURNAL   Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. doi: 
            10.1016/j.stemcr.2014.10.001.
  PUBMED    25454632
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. doi: 
            10.1016/j.stemcr.2014.10.001."
COMMENT     Sequence Label: TetO-FUW-mir124a
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(464..488)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(485..523)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    556..574
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    598..616
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    640..658
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    682..700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    724..742
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    766..784
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcgc ccttctaatc
       61 tagagcattg ttcgccggat ttgtccgcgg agcgaggggc caccgggggc cggggctcgc
      121 ctttttcccg cgggaggggt gccgcgacgg ccgggctccc gggccatttc catgagaaag
      181 gagcggcgga ggcgcctctc ttggcattca ccgcgtgcct taattgtata gacattaaat
      241 caaggtccgc tgtgaacacg cagaggggcc ctcagggctg cggcgggcgg cgagcggctg
      301 gaccgggtcg gggccgggct gcggcttgcg tggggtccta gcctgagggg cgcgaatgct
      361 ccagcttctt gtcgacaata agggcgaatt cggggccgcg gaggctggat cggtcccggt
      421 gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta
      481 aacgagctct gcttatatag gcctcccacc gtacacgcct acctcgaccc gggtaccgag
      541 ctcgactttc acttttctct atcactgata gggagtggta aactcgactt tcacttttct
      601 ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt
      661 ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac
      721 ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata
      781 gggagtggta aactcgactt tcacttttct ctat
//
