LOCUS       sequence-102238-       14606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MCP-VP64 and dCas9 in Yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14606)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 14606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC595
FEATURES             Location/Qualifiers
     source          1..14606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        3292..3996
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4024..4044
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4417..4566
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        4994..5660
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5530..5553
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             join(5670..5693,5694..5696,5697..8183,8184..8186,
                     8187..9770)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9783..9803
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9807..9827
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9831..9851
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(11153..11172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11154..11172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11179..11196)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11179..11195)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11188..11210)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11336..11791
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11423..11442)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11633..11654
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12147..12166)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12266..12288
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12326..12344)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12429..12533
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12534..12602,12603..13394)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12752..12771)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13565..14153
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14054..14073
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14307..14324
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14441..14462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14477..14482,14483..14500,14501..14507)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14515..14531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14520..14542
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14539..14555
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14539..14555
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        14576..14594
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccta aaacaagaag
     2521 agggttgact acatcacgat gagggggatc gaagaaatga tggtaaatga aataggaaat
     2581 caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg
     2641 ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc
     2701 atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct gggcgtgagg
     2761 ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc gctaaggggc
     2821 gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac cacgacaact
     2881 ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga gtatactaga
     2941 agaatgagcc aagacttgcg agacgcgagt ttgccggtgg tgcgaacaat agagcgacca
     3001 tgaccttgaa ggtgagacgc gcataaccgc tagagtactt tgaagaggaa acagcaatag
     3061 ggttgctacc agtataaata gacaggtaca tacaacactg gaaatggttg tctgtttgag
     3121 tacgctttca attcatttgg gtgtgcactt tattatgtta caatatggaa gggaacttta
     3181 cacttctcct atgcacatat attaattaaa gtccaatgct agtagagaag gggggtaaca
     3241 cccctccgcg ctcttttccg atttttttct aaaccgtgga atatttcgga tatccttttg
     3301 ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg
     3361 attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa
     3421 tagaacagat accagacagg acataatggg ctaaacaaga ctacaccaat tacactgcct
     3481 cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat
     3541 atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc
     3601 aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa
     3661 tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
     3721 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
     3781 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
     3841 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
     3901 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
     3961 acaatatttc aagctatacc aagcatacaa tcaactatct catatacaat gtctctcgag
     4021 atgcccaaaa agaaaagaaa agtgggtagt atggcttcta actttactca gttcgttctc
     4081 gtcgacaatg gcggaactgg cgacgtgact gtcgccccaa gcaacttcgc taacgggatc
     4141 gctgaatgga tcagctctaa ctcgcgttca caggcttaca aagtaacctg tagcgttcgt
     4201 cagagctctg cgcagaatcg caaatacacc atcaaagtcg aggtgcctaa aggcgcctgg
     4261 cgttcgtact taaatatgga actaaccatt ccaattttcg ccacgaattc cgactgcgag
     4321 cttattgtta aggcaatgca aggtctccta aaagatggaa acccgattcc ctcagcaatc
     4381 gcagcaaact ccggcatcta cggctccgga cgagccgatg ctctcgacga ttttgacctc
     4441 gatatgctgg gatccgacgc tctcgatgac ttcgaccttg acatgctggg gagtgacgct
     4501 ctggacgact tcgatctcga catgctgggc tccgacgccc tggatgactt cgatctggac
     4561 atgctcatca actgaagtgc ttttaactaa gaattattag tcttttctgc ttattttttc
     4621 atcatagttt agaacacttt atattaacga atagtttatg aatctattta ggtttaaaaa
     4681 ttgatacagt tttataagtt actttttcaa agactcgtgc tgtctattgc ataatgcact
     4741 ggaaggggaa aaaaaaggtg cacacgcgtg gctttttctt gaatttgcag tttgaaaaat
     4801 aactacatgg atgataagaa aacatggagt acagtcactt tgagaacctt caatcagctg
     4861 gtaacgtctt cgttaattgg atactcaaaa aagatggata gcatgaatca caagatggaa
     4921 ggaaatgcgg gccacgacca cagtgatatg catatgggag atggagatga tacctggccc
     4981 cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt
     5041 agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt
     5101 acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga
     5161 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag
     5221 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt
     5281 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac
     5341 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc
     5401 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa
     5461 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt
     5521 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat
     5581 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata
     5641 aacacacata aacaaacaaa atgctcgagg acaagaagta ttctatcgga ctggccatcg
     5701 ggactaatag cgtcgggtgg gccgtgatca ctgacgagta caaggtgccc tctaagaagt
     5761 tcaaggtgct cgggaacacc gaccggcatt ccatcaagaa aaatctgatc ggagctctcc
     5821 tctttgattc aggggagacc gctgaagcaa cccgcctcaa gcggactgct agacggcggt
     5881 acaccaggag gaagaaccgg atttgttacc ttcaagagat attctccaac gaaatggcaa
     5941 aggtcgacga cagcttcttc cataggctgg aagaatcatt cctcgtggaa gaggataaga
     6001 agcatgaacg gcatcccatc ttcggtaata tcgtcgacga ggtggcctat cacgagaaat
     6061 acccaaccat ctaccatctt cgcaaaaagc tggtggactc aaccgacaag gcagacctcc
     6121 ggcttatcta cctggccctg gcccacatga tcaagttcag aggccacttc ctgatcgagg
     6181 gcgacctcaa tcctgacaat agcgatgtgg ataaactgtt catccagctg gtgcagactt
     6241 acaaccagct ctttgaagag aaccccatca atgcaagcgg agtcgatgcc aaggccattc
     6301 tgtcagcccg gctgtcaaag agccgcagac ttgagaatct tatcgctcag ctgccgggtg
     6361 aaaagaaaaa tggactgttc gggaacctga ttgctctttc acttgggctg actcccaatt
     6421 tcaagtctaa tttcgacctg gcagaggatg ccaagctgca actgtccaag gacacctatg
     6481 atgacgatct cgacaacctc ctggcccaga tcggtgacca atacgccgac cttttccttg
     6541 ctgctaagaa tctttctgac gccatcctgc tgtctgacat tctccgcgtg aacactgaaa
     6601 tcaccaaggc ccctctttca gcttcaatga ttaagcggta tgatgagcac caccaggacc
     6661 tgaccctgct taaggcactc gtccggcagc agcttccgga gaagtacaag gaaatcttct
     6721 ttgaccagtc aaagaatgga tacgccggct acatcgacgg aggtgcctcc caagaggaat
     6781 tttataagtt tatcaaacct atccttgaga agatggacgg caccgaagag ctcctcgtga
     6841 aactgaatcg ggaggatctg ctgcggaagc agcgcacttt cgacaatggg agcattcccc
     6901 accagatcca tcttggggag cttcacgcca tccttcggcg ccaagaggac ttctacccct
     6961 ttcttaagga caacagggag aagattgaga aaattctcac tttccgcatc ccctactacg
     7021 tgggacccct cgccagagga aatagccggt ttgcttggat gaccagaaag tcagaagaaa
     7081 ctatcactcc ctggaacttc gaagaggtgg tggacaaggg agccagcgct cagtcattca
     7141 tcgaacggat gactaacttc gataagaacc tccccaatga gaaggtcctg ccgaaacatt
     7201 ccctgctcta cgagtacttt accgtgtaca acgagctgac caaggtgaaa tatgtcaccg
     7261 aagggatgag gaagcccgca ttcctgtcag gcgaacaaaa gaaggcaatt gtggaccttc
     7321 tgttcaagac caatagaaag gtgaccgtga agcagctgaa ggaggactat ttcaagaaaa
     7381 ttgaatgctt cgactctgtg gagattagcg gggtcgaaga tcggttcaac gcaagcctgg
     7441 gtacctacca tgatctgctt aagatcatca aggacaagga ttttctggac aatgaggaga
     7501 acgaggacat ccttgaggac attgtcctga ctctcactct gttcgaggac cgggaaatga
     7561 tcgaggagag gcttaagacc tacgcccatc tgttcgacga taaagtgatg aagcaactta
     7621 aacggagaag atataccgga tggggacgcc ttagccgcaa actcatcaac ggaatccggg
     7681 acaaacagag cggaaagacc attcttgatt tccttaagag cgacggattc gctaatcgca
     7741 acttcatgca acttatccat gatgattccc tgacctttaa ggaggacatc cagaaggccc
     7801 aagtgtctgg acaaggtgac tcactgcacg agcatatcgc aaatctggct ggttcacccg
     7861 ctattaagaa gggtattctc cagaccgtga aagtcgtgga cgagctggtc aaggtgatgg
     7921 gtcgccataa accagagaac attgtcatcg agatggccag ggaaaaccag actacccaga
     7981 agggacagaa gaacagcagg gagcggatga aaagaattga ggaagggatt aaggagctcg
     8041 ggtcacagat ccttaaagag cacccggtgg aaaacaccca gcttcagaat gagaagctct
     8101 atctgtacta ccttcaaaat ggacgcgata tgtatgtgga ccaagagctt gatatcaaca
     8161 ggctctcaga ctacgacgtg gacgccatcg tccctcagag cttcctcaaa gacgactcaa
     8221 ttgacaataa ggtgctgact cgctcagaca agaaccgggg aaagtcagat aacgtgccct
     8281 cagaggaagt cgtgaaaaag atgaagaact attggcgcca gcttctgaac gcaaagctga
     8341 tcactcagcg gaagttcgac aatctcacta aggctgagag gggcggactg agcgaactgg
     8401 acaaagcagg attcattaaa cggcaacttg tggagactcg gcagattact aaacatgtcg
     8461 cccaaatcct tgactcacgc atgaatacca agtacgacga aaacgacaaa cttatccgcg
     8521 aggtgaaggt gattaccctg aagtccaagc tggtcagcga tttcagaaag gactttcaat
     8581 tctacaaagt gcgggagatc aataactatc atcatgctca tgacgcatat ctgaatgccg
     8641 tggtgggaac cgccctgatc aagaagtacc caaagctgga aagcgagttc gtgtacggag
     8701 actacaaggt ctacgacgtg cgcaagatga ttgccaaatc tgagcaggag atcggaaagg
     8761 ccaccgcaaa gtacttcttc tacagcaaca tcatgaattt cttcaagacc gaaatcaccc
     8821 ttgcaaacgg tgagatccgg aagaggccgc tcatcgagac taatggggag actggcgaaa
     8881 tcgtgtggga caagggcaga gatttcgcta ccgtgcgcaa agtgctttct atgcctcaag
     8941 tgaacatcgt gaagaaaacc gaggtgcaaa ccggaggctt ttctaaggaa tcaatcctcc
     9001 ccaagcgcaa ctccgacaag ctcattgcaa ggaagaagga ttgggaccct aagaagtacg
     9061 gcggattcga ttcaccaact gtggcttatt ctgtcctggt cgtggctaag gtggaaaaag
     9121 gaaagtctaa gaagctcaag agcgtgaagg aactgctggg tatcaccatt atggagcgca
     9181 gctccttcga gaagaaccca attgactttc tcgaagccaa aggttacaag gaagtcaaga
     9241 aggaccttat catcaagctc ccaaagtata gcctgttcga actggagaat gggcggaagc
     9301 ggatgctcgc ctccgctggc gaacttcaga agggtaatga gctggctctc ccctccaagt
     9361 acgtgaattt cctctacctt gcaagccatt acgagaagct gaaggggagc cccgaggaca
     9421 acgagcaaaa gcaactgttt gtggagcagc ataagcatta tctggacgag atcattgagc
     9481 agatttccga gttttctaaa cgcgtcattc tcgctgatgc caacctcgat aaagtcctta
     9541 gcgcatacaa taagcacaga gacaaaccaa ttcgggagca ggctgagaat atcatccacc
     9601 tgttcaccct caccaatctt ggtgcccctg ccgcattcaa gtacttcgac accaccatcg
     9661 accggaaacg ctatacctcc accaaagaag tgctggacgc caccctcatc caccagagca
     9721 tcaccggact ttacgaaact cggattgacc tctcacagct cggaggggat gagggagctg
     9781 atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga
     9841 agagaaaggt atagaattct accggcggcc gccaccgcgg tggagctcta agcaaatagc
     9901 taaattatat acgaattaat attatgatta agtgtttacg tgagtgcgat atttttatta
     9961 ctatcttata cagttgtata tactctataa aatgagttgt ctattaatta acgcgatgaa
    10021 tgctttctgg gtttacctct ccaacaactc tagtttactt ctcaatacat tcaattgtat
    10081 ttgatttgtc aatacttcat cattaatcaa ttctatagtt ttgtttttct cgtttatttc
    10141 caaatttaat gcatcaattt tattattcaa tttgtcgttg attttggtta atgattttat
    10201 ggtttgatct ctggcattga ttgtttgtgt tagtttttca ttattgataa ttaaattatt
    10261 taagttagtt atcaactcgg tgttttcaag tttcaagttt tcaatttctt tagagtttat
    10321 tagatttgtc aaagtttctg aattgcttga ttggtcctgt agaagagtat ttgttgttgt
    10381 ggataattga ttcaattttt gagacaattg ctggaaggcg ttgaaatatc tagcatcaat
    10441 ctcatggttt ttttcccgag agtctcgtag attcaattgt tttaatatat cttgggacca
    10501 ctcttgattt gaactcatgg aaattaaact gggtgttgtg ttgtggtgta atgattgtac
    10561 cccctttgct tataattgtg tggccaccga agtcggtgat gctgtcgccg aagaagttaa
    10621 gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa
    10681 tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg
    10741 aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga
    10801 atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt
    10861 aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca
    10921 tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa aaaaatacaa
    10981 caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat accttcttga
    11041 accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga aacggcctta
    11101 acgacgtagg ccggccattt aaatgtttaa acggccacgc tggcccaatt cgccctatag
    11161 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    11221 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    11281 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
    11341 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    11401 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    11461 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    11521 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    11581 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    11641 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    11701 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    11761 aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
    11821 tgtgcggtat ttcacaccgc atagggtaat aactgatata attaaattga agctctaatt
    11881 tgtgagttta gtattaggat ctctaccctt ggcgaaaagt cctctgccaa caatgatgat
    11941 atctgatcca ccacttacaa cttcgtcgac ggttctgtac tgctgaccca atatgcgtat
    12001 atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga
    12061 atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt
    12121 gaaaaggtgg tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    12181 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
    12241 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
    12301 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
    12361 atgataataa tggtttctta gacgtcggcg cgccgaggac gtcaggtggc acttttcggg
    12421 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    12481 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    12541 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    12601 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    12661 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    12721 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    12781 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    12841 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    12901 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    12961 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    13021 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    13081 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    13141 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    13201 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    13261 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    13321 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    13381 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    13441 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    13501 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    13561 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    13621 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    13681 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    13741 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    13801 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    13861 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    13921 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    13981 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    14041 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    14101 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    14161 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    14221 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    14281 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    14341 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    14401 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    14461 attaggcacc ccaggcttta cactttatgc ttccggctcc tatgttgtgt ggaattgtga
    14521 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcggaatta
    14581 accctcacta aagggaacaa aagctg
//
