LOCUS       sequence-102249-        6837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complete genome of BoPyV1 isolate S22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Peretti A, FitzGerald PC, Bliskovsky V, Buck CB, Pastrana DV
  TITLE     Hamburger polyomaviruses.
  JOURNAL   J Gen Virol. 2015 Jan 7. pii: vir.0.000033. doi: 
            10.1099/vir.0.000033.
  PUBMED    25568187
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Virol. 2015 Jan 7. pii: vir.0.000033. doi: 10.1099/vir.0.000033."
COMMENT     Sequence Label: pBoPyV1-S22
FEATURES             Location/Qualifiers
     source          1..6837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     176..198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     190..207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     191..207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    223..322
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5031..5130)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(5726..5744)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5736..5783)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(5869..6457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5949..5968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6665..6682)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      join(6835..6837,1..84)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
       61 aacgctctcc tgagtaggac aaatccgccg ggagaacaac aacaattgca ttcattttat
      121 gtttcaggtt cagggggagg tgtgggaggt tttttaacgc tagcatggat gttttcccag
      181 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
      241 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
      301 actttgtaca aaaaagttgg cttacccggg gcctggcttc ccgccggatt ttcttcctca
      361 ttatctgatg gctctaattc ctcatcacaa tgcagatctt gctcatcaaa gccttgattg
      421 aattcttccc accactgttc ccactgtgag gtgccatatt ctggaacctt aaccagaaaa
      481 tcttaggtaa gtatatattt ttatttttta agcttatata tccaataatt aagaaatacc
      541 atacctggct agtagtggca cttgcagcaa tttttgcttt attatacaaa tacagaagtc
      601 ttttcattgc ttcttcattc cctcctaaaa ataaaatcag atttactgcc tgtaaacaag
      661 atttgcatgt acagatcaat actaaatata tttaccttta tctggatgat gcaccttgca
      721 tgccttcagg aatgcctttt tcaaagtatc tgcattgcca atatcagggg ttcctaagag
      781 ccccctaagc tcctcatatt cctcagatgt taattccatt ttcctctttt agggcgcgcc
      841 tcggaggcct gtccactact tctgtagaag aggttggagg cttaagcctc cggcctcagc
      901 tatatagaaa aaaaattaca gggtggctcc gcccagactc cgcccatctc tccgccccat
      961 ctctccgccc agtctccgcc taagggaaac cagtcccatc cgcaggagtc cctcaggaca
     1021 agaaacaata ggaacaacat ggccttacaa tgggtgtggc tgtgtcttag aaaaagttta
     1081 agaaacaaca aattaatgat gtgtagaata ttgattgaac tattgaaaat attgttagat
     1141 gttctggaca gcggggacta tgctgatggg ccctcagata accctggtac aggtattgct
     1201 aaggaggaga ttgtgaaatg cattgacttt cttaagatgc tggaggcagg agatgacgac
     1261 atagataagt atgggtttcc tgagctgctt gcggcacact ttgccaactg tttggcagtt
     1321 tgtttgaaag cagcgcggga aagcgacgcc atgttttgct gttctttgga agcaaacagc
     1381 tcagatttta actaaaggta ggccactaat gggtttattt tctaggattt tctaggatgg
     1441 gacaccatgg gagcccttct cacaatccta gctgaagttt ttgaattagc tactgctaca
     1501 gggctgtcag ctgaagctat cctcactgga gaggccttca ctactgccga actacttcaa
     1561 gcacatattg caaatcttgt tgaagttggt gaattgtctg tagcagaggc cttagcagct
     1621 acagaggtta ctagtgaagc tttcgaggcc ttgcaaagca tatcttctgt tcttcctaca
     1681 gcttttattg gtgttgctgc tacagaggga gctatacttg ggtcccttat tactttaact
     1741 gctacttcct cagcccttta tcctagtact tggaagcaca gcactcctag tgcaaacctt
     1801 aatcaggaaa tggctttggt tccttacata ggggatctag atatattttt tcctggggca
     1861 gaaactattt ctagatttgt gtacagtatt gatccttttc gctgggcctc atacctgtat
     1921 aatatagtag gaagagctgt atgggaacat ttatttagag aaacaaggag acaaatagct
     1981 tatcatacta cagatattgc tggaagaaca gcacaaagca tacatcatac tattgctaac
     2041 tttttggaaa atgttaggtg gactgtatct caccttggga caaacctgta ctctggttta
     2101 cacaattact atagacagct ccccccttta aatcccccac agtccagaga gttagcaagg
     2161 cgtcttgggg ttcctcaacc agatagacaa atatttgaaa aaggagagga gggaatgaag
     2221 catccagtct cagcagaata tgtagagaaa tatggagctc ctggaggtgc agagcaaaga
     2281 gttgctcctg attggctgct acctcttctt ttaggcctct acggtgatct tacccccgcc
     2341 tgggaagctg aagtagagga ggaagagaat gagcaggatg aggaagaata tgaacccccc
     2401 caaaaaagga ttaaaaggac agccaagtcc agttccaaag ttaataataa aaggggggat
     2461 agaagtgcta ggtctccgta caggaccaga cagcacaacc acaattgagc tgtttttgaa
     2521 cccaagaatg ggacaaagca ctgagtctga atattatggc ttcagtgaca accagagggg
     2581 atcaacctca agaacagatg aagacctaat tagtgcagaa ctgcccagat atagtttggg
     2641 agtggtacaa ttgcctttgc ttaatgaaaa acttactgat gatgttttgc ttatgtggga
     2701 agctgtaagt tgcaaaactg aggtcgttgg tgttaacact ttaacaacct gtcatggata
     2761 taagaaaagg tattcgccct ctgctggtca aggctcagca atgcctatag agggaataaa
     2821 ttaccatttc tttgctgtag gtggcgaacc acttgaaatc cagttcatat gtgaggattt
     2881 caaagccccc tatcatccca cagaaactat agtccctccc aaactcagca acaaatccca
     2941 ggtgctagat cctaccctca agggaattct agacaaagat ggtgtgtatc ctgttgaatg
     3001 ttggtgtcca gatccaagta aaaatgaaaa caccagatac ttcggaacat atacaggagg
     3061 tgttagcact ccacctgtgc tgcagttcac aaatactgtg acaaccattc ttcttgatga
     3121 gaatggcgta ggcccgcttt gtaaagctga caagctatat atcacagcag cagacatttg
     3181 tggctttctg actcagccga atgaccaaca gcaatttaga ggcctaccca ggtacatgtc
     3241 tgtgactctc aggaagagac ttgtgaagaa cccatacccc attgccagca tactcacatc
     3301 tttgtttacc aattccctgc cacctgttac tagccaagaa atggataaac aggttgagga
     3361 ggtcagaatt taccaagggg ttgagggcct accaggagat cctgacatgg tcaggtacat
     3421 caataagttt ggacaagagg aaacttgtat tccaaaatga ctttattatg gtactgcttg
     3481 ttacagttgt agatcaaagc tttatgaata aatcattttt ccagaatatt ttttccttgt
     3541 tcaatgtttt ctttcatggt taacaaccac atatctccca cctctgtctc aattctctct
     3601 ttccacttga ctacacattc ctgcagtttt tctgtgaaat catctacaga cctaaagtag
     3661 ataagtaaca agagccaggt gattccactc tgtaaatatc tttttttgcc cacttcctca
     3721 gtattcttaa gacttttata taagttataa ttatgcagta gttctacttt ttgatacata
     3781 cggattttga ctgttaaggg tatttcatag tcattacagg taacaattcc aggtgggaag
     3841 atctgagtaa ccttattttg atgttttctt tcaaggttta ctgggactgc gccatctaaa
     3901 tgatctctta aattatctaa attttcaaaa ccaattccag tctgaaggga ggatttactg
     3961 gatttcttac ctttgacatc ttcaaacaca actgtaaact gatcaattgc cattcctaat
     4021 tcaaatgcca gtctttcagg gggacaattt atatttaaat aagctccccc acacaggcct
     4081 ataaggcctg cagcaactgt agttttccca cagtttacag gtccctttaa gagcagatat
     4141 ctttgttttg gtttattttc aaccaccatc ttgatataat taaagataac atctggcata
     4201 ttgggatgta gggcattgag ccacataact gccgccattc ggtgggctaa atgctcctcc
     4261 ccccgagcgc caaacaaatc ctccatttct tgaaaaaggc cctgccacct ttgcactaat
     4321 aattcatttc tagttaatgt tttcatatct aatctccttt ttgctaacac agtatcacag
     4381 gcctgattac atatagtttt ctgagttttg ctgtcctgaa acagtaaagc atttaaggtg
     4441 tggtccttgg tatggtgtct tctatgctca aggcgtctgg gattgtcaca tactttacat
     4501 gcttcaggtg cagttgtaaa ttccaaatat aatgccatta ataatagagg gtctgtacat
     4561 ttaatactgc aagcaaattc agaaatgagt ttccaattta cagatttttc ttcttcttgc
     4621 acagggttaa agaaactact tggcagacct ccttgtatgt tttgggagat aagttcaaat
     4681 gtgcagcaaa cattataggc ctcctgaggt tttagaactc ccttggcttg tagaaagctc
     4741 acagtacatt ttttactaca cagattttcc attgcattta cccgatgttt tccaacagtg
     4801 ataatataca gaacagaatc accattatac atatacaagg caataaatgt acatttgtag
     4861 gctgcctgga tacaaggata taattctttt cctttctctg cagtagtaaa tattataaag
     4921 caattatatg ttctatttgt gaacagagca ttgcttacat attctttcaa aatctcagga
     4981 aagtcaggtg ctgttctagg tttcttgggt ggggtagctt gacttcccgg gtgcaacttt
     5041 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     5101 cactatcagt caaaataaaa tcattatttg ccatccagct gataagcaag taaaacctct
     5161 acaaatgtgg taaaatcgat aaggatccgg gctggcgtaa tagcgaagag gcccgcaccg
     5221 atcgcccttc ccaacagttg cgtacctaac caagttcctc tttcagaggt tatttcaggc
     5281 cctgcaggtt aattaacaaa aaaccccgcc ggggcggggt tttttgttac aattcttatc
     5341 ctcagtcctg ctcctctgcc acaaagtgca cacagttgcc tgctgggtct ctcagggcaa
     5401 actctctccc ccagggctgc tctccaatct ctgtcatggc agggccactg gcatccctga
     5461 agttggtgga gaccacctca ctccactcag catacagctc atccagtcct ctcacccaca
     5521 cccaagccag ggtgttgtca ggcaccacct ggtcctggac tgctgagatg aacagggtga
     5581 catcatctct gaccacacct gcaaagtcat cctccacaaa atctctggag aaccccaacc
     5641 tgtcagtcca gaactcaaca gctccagcca catccctggc tgtgagcact gggacagcac
     5701 tggtcaactt ggccatgatg gccctcctat agtgagtcgt attatactat gccgatatac
     5761 tatgccgatg attaattgtc aactactttt gaaaaattta attaagaaca tgtgagcaaa
     5821 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5881 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5941 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6001 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6061 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6121 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6181 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6241 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6301 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6361 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6421 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6481 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tggctagtta
     6541 attaacccac gtttcaacat gcacatccca gtaatttgga aacattttgt ttccaaagat
     6601 tcacttaaca ttggtttagc aacatgaagc ttggctgttt tggcggatga gagaagattt
     6661 tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg
     6721 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
     6781 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaa
//
