LOCUS       sequence-102289-        7224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complete genome of BoPyV2a isolate S22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Peretti A, FitzGerald PC, Bliskovsky V, Buck CB, Pastrana DV
  TITLE     Hamburger polyomaviruses.
  JOURNAL   J Gen Virol. 2015 Jan 7. pii: vir.0.000033. doi: 
            10.1099/vir.0.000033.
  PUBMED    25568187
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Virol. 2015 Jan 7. pii: vir.0.000033. doi: 10.1099/vir.0.000033."
COMMENT     Sequence Label: pBoPyV2a-S22
FEATURES             Location/Qualifiers
     source          1..7224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     176..198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     190..207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     191..207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    223..322
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5418..5517)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6113..6131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6123..6170)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(6256..6844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6336..6355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7052..7069)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      join(7222..7224,1..84)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
       61 aacgctctcc tgagtaggac aaatccgccg ggagaacaac aacaattgca ttcattttat
      121 gtttcaggtt cagggggagg tgtgggaggt tttttaacgc tagcatggat gttttcccag
      181 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
      241 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
      301 actttgtaca aaaaagttgg cccgcggatt caggtgagga tcccaaggtc tcgtcgcagt
      361 acagatccga gtcttgtgat tctgacctga actctctgga ataggcagta cctccaaata
      421 ctctggttcc tcctcccccg gtggctctgc ctcctccaga agacgagctt gtctttgcag
      481 acgaggtttg gctcccagac gctcccacat ctgaccgctg actagattct gaggcaaata
      541 taccggagcc tgtggtagag gttgaggttg tggagtctgg cgaggcggct ctgctaaatc
      601 tgctggaggg tcctccacgg gttggtttgg acgaggactg agactcagag aaataactgc
      661 catgctgctg ataccaccag gctttaaact gaggggtccc gtagataggt tcattttcct
      721 gcaaaaatag gagcaaatta gtaaagcttt ccagggtgat gtgaatcacc cccaggctgg
      781 tacttacaat aattaaatag gcgaagaagg tgcatatccg tgttcaagat aatttcttgg
      841 taccaatcaa aactttccca agtaggagga aagccaaacc acaaaaggta acaccggtag
      901 cagtagcagt ctccccaaat taggcatttt ttttcttggg tgtctttgat gtcctggtgt
      961 tgctgtgaca ggcggcaagt aatgcagcgg caaaaagagt acttactcca taagcaagga
     1021 ggccctctaa tgaatttgca ctgatttttc cccagatagt ccttcaaagt attaaaatcc
     1081 aaatcccaaa agaattttgc actgacctga taagaaaaaa agttaacttc tgcgcgcagc
     1141 ttagtgatct catcttgaaa ggaagtccaa agagaattta atttcaccat tttagagcca
     1201 tctccccctt tatcagggtg gtaaacaagg cacatctttt tatattgagc cttcatcatt
     1261 ggaatatttc cataacaatg atgagggatc tctaaaagat cacaaagctc tcttttttct
     1321 tgcctggtaa gtacagaatc catcttggta aaaataagaa gccaaaaaaa agggcgcgca
     1381 gcctgtcaca gcaagatata ttaatggaag cctcgcgaga gcctctcttc ttatttttga
     1441 aaaggtaaga ggcaagaagc ctccaagcct ccaactattt tttaaaaaaa ggagaggcct
     1501 ctgactaatt atgaaagggc ggtacggaaa cttggctaaa atatagcacc ttggcagcta
     1561 tgcaaaggcg ggttgcctag taacaaaacc acaggccttc ccctgcggtt acagattctg
     1621 gactgtgata ggggaagttg ctgatgcgtg cagtgttgat gacactattt cctgtgacac
     1681 agacgtgact agtgacactt ttatttcctg ttttagctca aattgctgct gcgttgcctt
     1741 agcgacggtt acttagctac ggctctccgc ttctgctata aagtaagagc ttttattttg
     1801 ctttctttta ggcgtgatgg gggctttcat atcactgcta ctaaatgtgg gagctttggc
     1861 tgctgaactc agtgctgaga ctgctataag tgtggaatct atactaactg gtgaagcttt
     1921 ggctgctttg gaggctgaac ttgctagtgt tatgacaatt caaggcataa gcggcattga
     1981 ggccttgacc caacttggct ttacagcaga gcaattttct aatttttctc tggtttcttc
     2041 tcttgtaaat caagctctat cttatgcctt ttattttcaa actgtatctg gggcagctgc
     2101 tctagtagag gcagggatta gactgggaat aggggacaga agtgtagtgg aatctcattc
     2161 tgatgtaggc ctcaacccta gtggacatat tataaggcag tttgctaagg gatttaagat
     2221 tgacccttta aaatggagtc aatctttgat ccacaatatt gggaggacag tttttgaaag
     2281 tgtaaatgcc catgatagga aaacacagtc aatggctcag aattatgcta gattaataga
     2341 gatgggaagg tggtatgtag agactgatga aagtataaca gctggaaatg actcaggaat
     2401 ttcagtggct gtttacactg ctcctggtgg ggctgaacaa agagtgacac ctgactggct
     2461 ccttccttta attttaggtc tcagtggaga cactacacct acttggaaag cagattttaa
     2521 gataaatggc tcctaaaaga aagagagttt gtaaaccctg taagatgaca agtgcttctt
     2581 ctacatcaac atgtaataga gacccttgtg gaaggccttg tccaaaggtg gatccagttc
     2641 ctaaattgct agtaaaagga ggtgttgagg tacttaatgt tgaatcaggc ccagatgcta
     2701 ttacccaaat tgaactttac cttgacccta gaatgggggt caatgcaatt gacataccta
     2761 cttatagtga taattatggg tacagttata aaattgctcc ctccttaact gatcccagta
     2821 ctcctaatcc agaaaatctg cctacttaca gtgtaggcag agttgcactg cccatgttaa
     2881 atgaagatat gacctgtgat accctcttaa tgtgggaagc agtgtcagtg aaaacagagg
     2941 ttgtaggggc caactccttc attaaccagc acgcatataa acaaaaagaa atttttactg
     3001 gcggggctgg cattcctata gagggtgctc agttccatat gtttgcagta ggtggtgaac
     3061 cactgcaatt acaagggata gtagccaatt ataaaaccaa ataccctaag ggcagtcata
     3121 tgcccattag tatagaaaca gttacaggca aggcaatgac tcctaagaat caaggccttg
     3181 atcccactgc taaagccttg ctggacaagg atggctacta tcctgtggaa atctggaccc
     3241 cagaccctgc caggaatgag aacagtaggt attatggctc ttatactgga ggggaaagaa
     3301 cacctcctgt ccttcagttt actaacacct taaccacagt ccttcttgat gagaatggga
     3361 ttggtccatt atgtaaaggt gacgggctct ttctttccgc tgcagacctc tgtggttatt
     3421 attttaaaga tgagtctatg agatggcgtg gactccccag atattttaat gtaactctca
     3481 gaaagagaag agtgaaaaac ccatatccag tttcgagttt actagccaga tttttcactg
     3541 gtcttatacc aaaaatcaaa ggtcaggaaa tggagggaga aaagagtcaa gtggaagaag
     3601 tgaggatcta tgatggaaca gaacctgtac caggagaccc agatcttatc aggtatatag
     3661 acaaatttgg cacagacaga acagaagttc caaagtaaaa attttattgt gactgtgtaa
     3721 atcttccaga atcatctgta ctttcctcct caataaagtc ttttaatgga tccataccct
     3781 tctcaatgtt ttcaatcatc acacagaatt tttcattacc tatttcagaa tttaaaatac
     3841 ttttccaact agcaacttct tctctaatac tactgtgaaa cttagaagca ggacagagcc
     3901 aaattaatgc taagagaagg gttaggcctt gctgcagaat cctgtgctta cttaaatcct
     3961 tgttagcatc taaagctttt tttaatactt ttctaggttc aaatttcaga gtataagcaa
     4021 accttgcaag cagagtctga gggaatacat attcattggc agtgacaata catggaggaa
     4081 aaatttgagt cctcttgtta acatgctttc tttccaagtt gacagaaaca gctccatcca
     4141 gatgttctct caggttgtca aggttgtgaa ttccttgtcc cggctggaga gttttattga
     4201 gggaggtctg tccctttaca tcttcaaaca caacagcaaa gtggtcaata gcacatccaa
     4261 gttcaaaatt cagcttatca gcaggacaat taacattcag gctttttccc tcaattaaat
     4321 ctaagatagc agcagcaaag ctagttttcc cactattaat gggtccaata aagagagagt
     4381 ttctgtgctt tggacagttt tcagtcagta attgcaacac tctaacaagc ttttcttcaa
     4441 agtcactaaa taagcagcaa taccaagcaa cccccgccat gtgctctaat aaggggatag
     4501 tatgtaattc tcttaattta cttaattgct tcttaaattt ctctgccaaa agttcttctc
     4561 tgctactctc caatatttta agccttttct ttgctaaaac tacatctgct gcttgctggc
     4621 aaatattttt ttgagcttta gcatttttaa aaagctttgc attattatgc tgcttttcat
     4681 gatctttatg agctttaagc cccttcccat ggcactttgc acatggaaag ggattggcaa
     4741 aatcaagata gtgagccatt ataattaaag gatcctctaa ttcatatgtt tctgcaaact
     4801 cagcaatggc agtccaagac acactctctg ccttctcatt ttttgaatca aattcataag
     4861 accagacccc attcctattt tcctctaaaa gtgcaaaagg ttcattacat aatgctctat
     4921 aaaattctgg acttttgtta acacctttta caattaaaaa gcttatagta caaaacatgc
     4981 tacaataatt cttaacagca gatactctat gtttagtaag agtgatgagg aacagaattc
     5041 cagctaaaga accatcagga gccttatatt tatgcctact tttaaagtct actttaaact
     5101 tatcacattt ctcatataat aaagttactt tttccaaagt ggaatagaca gcaaaagcac
     5161 ttactgtttt attgctatac acagcgtgag agagataatc aagcaggcag ctaggaaaat
     5221 cagtaggatg atggaaactc tcattctttt ttgtctttgg tggagtagag tcaaatgttc
     5281 ttgaagatcc tgcactacta tctccatcat caaatgctct ctttctagct gctcccggag
     5341 gcatctttct cggtttctgg ggtgttgagg tggagggctg ggaggtactg gaggagggac
     5401 cgtcatcctc tcccgcggtg caactttctt gtacaaagtt ggcattataa gaaagcattg
     5461 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     5521 tccagctgat aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgggct
     5581 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgt acctaaccaa
     5641 gttcctcttt cagaggttat ttcaggccct gcaggttaat taacaaaaaa ccccgccggg
     5701 gcggggtttt ttgttacaat tcttatcctc agtcctgctc ctctgccaca aagtgcacac
     5761 agttgcctgc tgggtctctc agggcaaact ctctccccca gggctgctct ccaatctctg
     5821 tcatggcagg gccactggca tccctgaagt tggtggagac cacctcactc cactcagcat
     5881 acagctcatc cagtcctctc acccacaccc aagccagggt gttgtcaggc accacctggt
     5941 cctggactgc tgagatgaac agggtgacat catctctgac cacacctgca aagtcatcct
     6001 ccacaaaatc tctggagaac cccaacctgt cagtccagaa ctcaacagct ccagccacat
     6061 ccctggctgt gagcactggg acagcactgg tcaacttggc catgatggcc ctcctatagt
     6121 gagtcgtatt atactatgcc gatatactat gccgatgatt aattgtcaac tacttttgaa
     6181 aaatttaatt aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6241 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6301 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6361 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6421 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6481 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6541 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6601 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6661 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6721 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6781 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6841 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6901 ttaagggatt ttggtcatgg ctagttaatt aacccacgtt tcaacatgca catcccagta
     6961 atttggaaac attttgtttc caaagattca cttaacattg gtttagcaac atgaagcttg
     7021 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa
     7081 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca
     7141 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
     7201 gagtagggaa ctgccaggca tcaa
//
