LOCUS       sequence-102291-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Sondheimer N, Lindquist S
  TITLE     Rnq1: an epigenetic modifier of protein function in yeast.
  JOURNAL   Mol Cell 2000 Jan;5(1):163-72.
  PUBMED    10678178
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2000-01"; volume: "5(1)"; pages: "163-72"
COMMENT     Sequence Label: RNQ pROEX-HTb
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    90..106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     95..117
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             139..156
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             178..198
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat
       61 taatcatccg gtccgtataa tctgtggaat tgtgagcgga taacaatttc acacaggaaa
      121 cagaccatgt cgtactacca tcaccatcac catcacgatt acgatatccc aacgaccgaa
      181 aacctgtatt ttcagggcgc catgggatcc atggatacgg ataagttaat ctcagaggct
      241 gagtctcatt tttctcaagg aaaccatgca gaagctgttg cgaagttgac atccgcagct
      301 cagtcgaacc ccaatgacga gcaaatgtca actattgaat cattaattca aaaaatcgca
      361 ggatacgtca tggacaaccg tagtggtggt agtgacgcct cgcaagatcg tgctgctggt
      421 ggtggttcat cttttatgaa cactttaatg gcagactcta agggttcttc ccaaacgcaa
      481 ctaggaaaac tagctttgtt agccacagtg atgacacact catcaaataa aggttcttct
      541 aacagagggt ttgacgtagg gactgtcatg tcaatgctaa gtggttctgg cggcgggagc
      601 caaagtatgg gtgcttccgg cctggctgcc ttggcttctc aattctttaa gtcaggtaac
      661 aattcccaag gtcagggaca aggtcaaggt caaggtcaag gtcaaggaca aggtcaaggt
      721 caaggttctt ttactgcttt ggcgtctttg gcttcatctt tcatgaattc caacaacaat
      781 aatcagcaag gtcaaaatca aagctccggt ggttcctcct ttggagcact ggcttctatg
      841 gcaagctctt ttatgcattc caataataat cagaactcca acaatagtca acagggctat
      901 aaccaatcct atcaaaacgg taaccaaaat agtcaaggtt acaataatca acagtaccaa
      961 ggtggcaacg gtggttacc
//
