LOCUS       sequence-102300-        7217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complete genome of BoPyV2b isolate S24.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Peretti A, FitzGerald PC, Bliskovsky V, Buck CB, Pastrana DV
  TITLE     Hamburger polyomaviruses.
  JOURNAL   J Gen Virol. 2015 Jan 7. pii: vir.0.000033. doi: 
            10.1099/vir.0.000033.
  PUBMED    25568187
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Virol. 2015 Jan 7. pii: vir.0.000033. doi: 10.1099/vir.0.000033."
COMMENT     Sequence Label: pBoPyV2b-S24
FEATURES             Location/Qualifiers
     source          1..7217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     176..198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     190..207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     191..207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    223..322
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5411..5510)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6106..6124)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6116..6163)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(6249..6837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6329..6348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7045..7062)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      join(7215..7217,1..84)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
       61 aacgctctcc tgagtaggac aaatccgccg ggagaacaac aacaattgca ttcattttat
      121 gtttcaggtt cagggggagg tgtgggaggt tttttaacgc tagcatggat gttttcccag
      181 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
      241 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
      301 actttgtaca aaaaagttgg cgaattcctt gtcccggttg cagagtttta ttaagactgg
      361 tttgtccctt tacatcttca aatacaacag caaaatggtc aatagcacag ccaagttcaa
      421 agtttagctt atcagcaggg caattcacat ttaagctttt tccctcaatt aaatctaaga
      481 tagcagcagc aaagcttgtt ttcccactgt taataggacc aataaaaagg caatttctgt
      541 gctttggaca gttttcagtc aaaagttgca acactttaac aagcttttct tcaaagtcat
      601 taaataagca gcaataccag gccactcctg ccatatgctc taataaaggt atagcagata
      661 attcttttaa tttacttaat tgctttttaa atttctctgc caaaagctct tctctgctac
      721 tttctaatat ttttaatctt ttctttgcaa gcacaacatc tgcagcttgc tggcatatgt
      781 ttttttgagc ttttgcattt ttaaacagct ttgcattgtt atgttgcttt tcatgatctt
      841 tgtgagcttt tagtcctttt ccatggcact tagcacatgg gaaaggatta gcaaaatcaa
      901 gataatgtgc cataataatt aaaggatctt ctaattcata agtttctgca aattcagcaa
      961 tagctgtcca agacacactt tcagcttttt catttttaga gtcaaattca taggaccata
     1021 caccattcct attttcctct aataattgaa aaggctcatt acataatgct ctgtaaaatt
     1081 caggactttt gttcacccct tttacaatta aaaagcttat tgtacaaaac atgctacaat
     1141 agtttttaac agctgaaact ctatgtttag ttaaagtaat taaaaacaga atgccagcta
     1201 aggagccatc agggcttctg tatttgtgcc tgcttttaaa atcaacttta aacttatcac
     1261 atttttcata taataaagtt attttttcta aagtagaata tacagcaaaa gaacttactg
     1321 ttttattgct atacacagcg tgagagagat aatcagacag gcaactagga aaatcagcag
     1381 gatgatgcat attctcattc ttttttgtct ttggtggagt actgtcaaat gttcttgaag
     1441 atcctgcact actatctcca tcatcaaatg ctctctttct agctgctcct ggaggcgcct
     1501 ttctcggttt ctggggtgtt gaggtggtgg gctgggaggc actggtggag ggaccgtcat
     1561 cctctcccgt ggattcaggt gaggaaccca aggtctcgtc gcagtacaaa tctgagtctt
     1621 gtgtttctga cctgaactct ctggaatagg cagcacttcc aaatactccg gttcctcctc
     1681 cgctgggggc tctgcttctt ccagaagacg aggttgtctt tgcagacgag gtctggctcc
     1741 cagccggtcc cacatctgaa tgctgacttg attcggtaaa tataccggat cctgtggttg
     1801 aggttgaggt tgtggagtct ggcgaggtgg ctctgctgaa tcttctggag ggtcctccac
     1861 gggacggttt ggacgtggac tgcgactcag agaaaaaact gccatgctgc tggtaccacc
     1921 aggttttaaa ctgaggggtc ccgtagatag gttcattttc ctgtgaaaat aggagcaaat
     1981 tagcaaagct ttccaggttg atgtgaatca cccccaggct ggtacttaca ataattaaat
     2041 aggcgaagaa ggtgcatatc cgtgttcaaa attatttctt ggtaccaatc aaaagtttcc
     2101 caagtgggag gaaagccaaa ccacagaagg taacaccggt agcagtagca ttctccccaa
     2161 attaggcatt ttttttcttg agtatctttg atatcctgat gttgctgtga caggcggcat
     2221 acaatgcaac ggcagtaaga gtacttactg tggaggcagt ttggccctct taggaatttg
     2281 cactgatttt ttcccaggta ttccttcaaa gtgttaaaat ccagatccca aaaaaatttt
     2341 gcactgacct gatatgaaaa aaagttaact tctgcacgca gttttgttac ttcatcttga
     2401 aaggaggtcc atagagagtt taatttcacc attttagctc catccccccc cttatcagga
     2461 tggtaaagca agcacatctt tttatattgt gccttcatca ttggaatatt gccatagcaa
     2521 tgatggggga tctctaaaag atcacaaagc tcccttttct cttgcctgga aagaatggaa
     2581 tccatcttca cagaaatctt tgcaaaaaaa gggcgcgcag cctgtcacag caagatatat
     2641 taataagaag cctcgcgaga gcctctcttc ttattttcaa aaaagtgaga ggcaagaagc
     2701 ctccaagcct ccaactattt tttaaaaaaa ggagaggcct actgactaat taagagaggg
     2761 cggcacagga aacttggcag attcagtgca ccttggcaca tttgcaaagg cgggttgcct
     2821 agtaacaaaa ccacagccct tcgcctgcgg tcagagattt ctgcatgtga tagaggaagt
     2881 ggctgatctc tgcatatttt tcagactcat ttcctgtgac aaagcctgtg actagtgtca
     2941 cttttatttc ctgttttagc tcaaattgct gctgcgttgc cttagcgacg gtttcttagc
     3001 tacggctctc cgcttctgct ataaagtaag agcttttatt ttgctttctt ctcttttagg
     3061 atgggggctt tcatatcact gcttcttaat gtgggcgctt tggctgctga actcagtgct
     3121 gagactgcta taagtgtgga atctatacta actggtgaag ctttggctgc tttggaggct
     3181 gaaatagcaa gtgttatgac aattcaaggc atcagtggca ttgaagcctt gactcaactt
     3241 ggctttacag cagagcaatt ttctaatttt tctttagtat cttctcttgt aaatcaggca
     3301 cttcagtatg ctttttattt tcaaacagtt tctggtgctg ctgcattagt agaagcagga
     3361 ataaaatggg gtttaggaga aagaagtgta gtagatgcta gtgatgtagg cctcagtcct
     3421 aatggccgcc tgttaaggga gtttgcaaaa ggatttaaga ttgatccttt taactggagt
     3481 caatcattaa ttcacaatat tggcagaact atttttgaaa agataagtgc tcataataaa
     3541 attactcaag ccacagccca aaattatgct agattaattg aaatgggccg gtggtatgta
     3601 gaaagtacag atagtgtgac agctggaaat gactcaggaa tatcagtagc tgtttatcct
     3661 gctcctggtg gggcagaaca aagagcaact cctgactggc tccttccttt aattttaggc
     3721 ctcagtggag acactacacc cacttggaaa gctgatctag aaataaatgg ctcctaaaag
     3781 aaaaagggtt tgtaagccct gtaaaccaat ggcttctact tcagtatgta atagagaccc
     3841 ttgtggaagg ccttgcccta aagtggatcc tgtacctaaa ttgctagtaa aaggaggagt
     3901 ggaagttctc agcgtagagt caggcccaga ctctattacc cagattgaac tttaccttga
     3961 acctagaatg ggggtaaatg ctccagacat tcctacttac agcgataatt atgggtacag
     4021 ttataaaatt gcaccctctt taagcaatcc cagtactcct aatccagaaa atctgcctac
     4081 ctacagcgtg ggtagagtgg cactgcccat gcttaatgaa gatatgacct gtgataccct
     4141 cttaatgtgg gaagcagtat cagtgaaaac agaggttgtt ggtgccaact ctttcattaa
     4201 ccagcatgca tataaacaaa aagaaatttt ttcaggaggt gctggcattc ctatagaggg
     4261 agcacaattc catatgtttg cagtaggtgg tgaaccactg caattacaag gcatagttgc
     4321 caattataaa acaaaatacc ctaagggcag ccatatgcct attactatag aaacagttac
     4381 agggaagcct atgacaccta aaaaccaagg ccttgatccc acagcaaaag ccttgctgga
     4441 taaagatggg tattatccag tagaagtttg gaccccagac ccagcaaaaa atgaaaatag
     4501 cagatactat ggttcttaca caggaggtga aaggacaccc ccagtcctcc agtttaccaa
     4561 cactttaacc acagtgctac ttgatgaaaa tggaattggt ccattatgta aaggtgacgg
     4621 gctctttctt tccgctgcag acctttgtgg tttttatttt aaagatgagg ctatgaaatg
     4681 gagaggttta cccagatatt ttaatgttac tcttagaaaa agaagagtaa aaaacccata
     4741 tccagtttca agtttactgg ccagattttt tacaggccta ataccaaaag ttcaaggtca
     4801 gcagatggaa ggggaaaaga gtcaagtaga agaggttaga atttatgatg gcactgaacc
     4861 tattcctggg gacccagatc ttatcagata cattgacaaa tttggcacag acagaacaga
     4921 tcttccaaaa taaattttat tgtgactgtg tgaaccttcc agaatcatct gtatttgcat
     4981 cctcttcctc aataaaatct ttcaaaggat ccaaaccttt ttcaatgttt tcaatcatta
     5041 tacaaaattt ttcatttcca atttcagaat taagtatgct tttccaggta ctgatatcct
     5101 ctctaatgct actatgaaac ttatgagaag ggcaaagcca tattagtgct aaaagcagtg
     5161 ttaggccttg ctgtaaaatc ctatgctttc ctaagtcttt attagcttct aaagcttttc
     5221 ttagcacttt tctaggttca aattttaaag tataagcaaa tctagcaagt aaagtttgag
     5281 gaaatacata ttcattagca gtaacaatgc aagggggaaa tatttgagtc cttttgttga
     5341 catgttttct ttccaagttg actgaaacag ctccatccag atgctctcgc aggttgtcaa
     5401 ggttgtgaat tcgcaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     5461 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     5521 gataagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgg gctggcgtaa
     5581 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgtacctaac caagttcctc
     5641 tttcagaggt tatttcaggc cctgcaggtt aattaacaaa aaaccccgcc ggggcggggt
     5701 tttttgttac aattcttatc ctcagtcctg ctcctctgcc acaaagtgca cacagttgcc
     5761 tgctgggtct ctcagggcaa actctctccc ccagggctgc tctccaatct ctgtcatggc
     5821 agggccactg gcatccctga agttggtgga gaccacctca ctccactcag catacagctc
     5881 atccagtcct ctcacccaca cccaagccag ggtgttgtca ggcaccacct ggtcctggac
     5941 tgctgagatg aacagggtga catcatctct gaccacacct gcaaagtcat cctccacaaa
     6001 atctctggag aaccccaacc tgtcagtcca gaactcaaca gctccagcca catccctggc
     6061 tgtgagcact gggacagcac tggtcaactt ggccatgatg gccctcctat agtgagtcgt
     6121 attatactat gccgatatac tatgccgatg attaattgtc aactactttt gaaaaattta
     6181 attaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6661 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6901 attttggtca tggctagtta attaacccac gtttcaacat gcacatccca gtaatttgga
     6961 aacattttgt ttccaaagat tcacttaaca ttggtttagc aacatgaagc ttggctgttt
     7021 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     7081 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
     7141 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
     7201 gaactgccag gcatcaa
//
