LOCUS       sequence-102301-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen bicistronic lentiviral vector allows for simultaneous 
            expression of a transgene and EGFP marker to facilitate tracking of 
            transduced cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    34..585
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    36..603
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(195..214)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     424..441
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     misc_feature    652..769
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cggtgaacag ctcctcgccc ttgctcacca tggtattatc atcgtgtttt tcaaaggaaa
       61 accacgtccc cgtggttcgg ggggcctaga cgttttttaa cctcgactaa acacatgtaa
      121 agcatgtgca ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct
      181 tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca actatccaac
      241 tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt
      301 ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag
      361 acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca ttcaacagac
      421 cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag aagacagggc
      481 caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat aacatataga
      541 caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg gggggggggc
      601 ggaattcctg cagcccgtag tttaaacctc gaggctagtc tcgtgatcga taaaattttg
      661 aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac
      721 tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaag ttaaccgata
      781 ccgtcgagat taattaaatt taaatgtcga aattcctcac gacacctgaa atggaagaaa
      841 aa
//
