LOCUS       sequence-102306-        6999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complete genome of BoPyV3 isolate S22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Peretti A, FitzGerald PC, Bliskovsky V, Buck CB, Pastrana DV
  TITLE     Hamburger polyomaviruses.
  JOURNAL   J Gen Virol. 2015 Jan 7. pii: vir.0.000033. doi: 
            10.1099/vir.0.000033.
  PUBMED    25568187
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Virol. 2015 Jan 7. pii: vir.0.000033. doi: 10.1099/vir.0.000033."
COMMENT     Sequence Label: pBoPyV3-S22
FEATURES             Location/Qualifiers
     source          1..6999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     176..198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     190..207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     191..207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    223..322
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5193..5292)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(5888..5906)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5898..5945)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(6031..6619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6111..6130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6827..6844)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      join(6997..6999,1..84)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
       61 aacgctctcc tgagtaggac aaatccgccg ggagaacaac aacaattgca ttcattttat
      121 gtttcaggtt cagggggagg tgtgggaggt tttttaacgc tagcatggat gttttcccag
      181 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
      241 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
      301 actttgtaca aaaaagttgg ctgaattctt cccaccattg ctcccagcct tcttcaccat
      361 atttctataa aacaaacaat tagtgccttt acatgctacc ccccaggtgg aaaatcaaga
      421 atgcacatac ctagtataag gtgatttttc cccataggcc aagttcatcc atggtgctat
      481 tgaaaaggat acagctccac cagtaatgag cagcccagtc tcttcttaat ccaaaccaaa
      541 ctaaatagca attgaagcag taacattctc cccatgtaat gggtcttctc caggctttta
      601 gctttttttg atggatttga cttagcacac atgtaatgca tgtgcaggct ctcatcccaa
      661 acacaacaca gtgggaattt ctcttgatct tagagcccca gagaggcccc aacatctctc
      721 ccaaagtagg ataatcagaa tcccagaaaa aacttactgg tccagagaag aatccgggat
      781 cagactcatg cacagactcc agcttagctt gagctttagc ccagagatca tttaatctgg
      841 ccatggtctg attatcccca cctttatctg ggtggtattt aatcctggcc tccctgagct
      901 ttttcttcat taagggcata ttcccccaac aggtgggggg cagcccaaga agccccaaca
      961 gctcctttgc ttcttctctg gctagaaccc gatccataat tgcaggctgc accctttctc
     1021 tcaactgagg aggaggcaag gaaggccacc aagccacctc tatatataaa aaaaagtagg
     1081 cctgcatgtc attagttgct gggcaacctc aggccatttt ggcgggctgc cacaggatgt
     1141 gggagggtga cttaaaggac gttgcctagg agactgccgc cagtcacatg aaaggcgccg
     1201 gcggaaaacc acctggggcc tcttacttcc tctgtaagta acttattttt ctgttaattt
     1261 ctttagttaa ctctttacta cctttaattt aggcctactt ttttatattt tcagaaaaac
     1321 cctgtgttgt tcctgtcttt ggtggtggtt ggttaactac tggtaagatg ggcatcttac
     1381 tgtctttacc tgagattata gctgccgctg aagtgggctg ggcagaagct gtggccattg
     1441 ccggggggga agctgccatc ttctctggtg aaggagccgc tattctagag agcctgttaa
     1501 ctgcgggggc actaacaggc gaggctacca cagcacttgg aatctctgac gcagctgcca
     1561 ttacattgac agcttttcca gagctaacca agacatttct tactctgcag accacacttg
     1621 ctgctgctag ctttgctgga ggtgttgcct attccatcaa tcctggggag cgggtgttca
     1681 agagtattga aggaccaggt ggactcgggc cccaacttag aggaaacatg gcacttcaac
     1741 tgtggtttcc acaaacgggg ctgtggcaaa actatggtgt cagcctcccc gactggtgga
     1801 gtagcttgct cagccaattg cctgatccct acacaatttt gcaggacatt gccagaggaa
     1861 tctggacctc ctactataga gctggacaag aaattgtcag aagaacagtt ggaaatcaaa
     1921 tcagaggatt tgtaggctct gttcaagaga acctgctcca aactggcagg gctattgtag
     1981 aaagggcccc tgatccagtt acaggcctca ttaatgtctg gaattgggct actgagcatc
     2041 aaagacaatg ggaaactcaa gctctcctag aaggacagcc tgtgtttggg agggagaccc
     2101 aatgggagag tcaaaacctc ccaatagatg ggggaaataa ccaaagggat ggatggcatt
     2161 ggggagggca atgggttact atgcctggca gcaatctagg tgcctataca gtgcctcagt
     2221 ggatgttgta tgttctagag gaagtggaaa aggacctgac tgcccatcat ggcaacatgc
     2281 ggagcaggaa gaggcaaggt gaccctgcca agacaaataa aaaaaggagg cgctgaggtc
     2341 ctggatacag tccccttgtc tgagaccacc cagtatgctg tagagtttgt gaacaagcca
     2401 acctttctga agaccaatgg ggacccagaa tacagatcta gaggcctgca atacctgctt
     2461 gatgacccga ctgtaggtgt tcagggtgct ttgtgctact cactcggggt aattgacctg
     2521 cctgaaattc ctaatgctat gtgtgaagat tctgtaattg tttgggaagc ttatagagtg
     2581 gagactgagc ttatcactct gcctgtgctg gggtctgggg gctatgtttc tgctacaaaa
     2641 actattgcaa gtgttgaagg tccacaaatg tatttttggg ctgttggggg cagtcctctt
     2701 gatgttgtgg ggtttaatcc tgacccagat gggacctaca cttttgcagc tgagcttgct
     2761 gcccctaagg gcactatatc tgttgcaggc cctggtgcaa ctaaaagcca ggttactact
     2821 cctaaatata cagtagaggg ctgggctgca gatccttcca tgaatgataa taccaaatat
     2881 tttggaagaa ttataggagg atcccagact ccacctgtaa tcacctatgg aaatggctca
     2941 accacacctt tggttgatga atatggagtg ggtccattgt gcctacatgg cgtctgttat
     3001 ctgacctctg ttgatctaat gggaactgtg ggctggcctg gacaggcaac acttcctgat
     3061 ggaggagtta agaaaagagc cctgcagact gctgcaggca ggtttttcag agtccacttc
     3121 agacagagaa gggttaagaa tccctggacc atggagcaaa tgctgagtca atttctcagg
     3181 cctaaagctc ctgtggttac tggggctcaa acagaggtca gtgaagtgac aatgacccag
     3241 gacaaaggtc cttccagtat tcctcccacc cttgaggctt ctgtgggtta tggtcaagct
     3301 atttttaatg ttgtggatgg ccagcttgtc tacccagatg gacagggggg gagcataaat
     3361 ataggaacta gaacacaaat gtaagccaaa gcaacagttt attgtatgca gtatctgcag
     3421 gattatgctt ttgctatgct tttttataca gaattaaaag aatttgcatc agaaaaacca
     3481 gtgtctgctg tactctgtgt aggccttgtt tgctcctctt cttcttcttc ctctccatca
     3541 taaacacaga tgccctccaa tgcccccttg ccatcctgga tgtttatcct catatctaca
     3601 aatgaggtaa aagaaatcca tgcttcaaag acagttttcc agtgcacaac ttgctcttgc
     3661 actttggcca taaacatgct aacaggttca ttccatacaa gcaatgcaag cagtgttaca
     3721 gggttggtaa ggagtctttt cctcaccaga aaatcatcct ttaacagggt tttgctaagg
     3781 ttaggcctat gcctgaattt aatggttttg gcaattctga ccttaactgt tttggggact
     3841 tcatagtcat ttgaggttat gatacccggg ggccacactt gctgcacttt gtttacatgt
     3901 ttcttctcca aattcactgc tacagcacca tctaaatggt ctctcatgtt gtccaggttg
     3961 gtgaatccac agccatgggg gagggtagga tctgcagctg attgtccttt aacatcttct
     4021 aagagaactg caaacttgtc tatcccacac cctaattcaa aaggcaattt ttcagcaggc
     4081 acattaatat ttaatgatgc accttgcact agatttatta tggctgctgc aagagtagtc
     4141 ttacctgaat taagtgggcc aatgaaatgc acatatcttt tctttggggt gttattaacc
     4201 agtatttcta atatggacac tacctgttca gagcaatcag gtatcagttc catgagccaa
     4261 gccactcctg ctaaagtttc tgcaatttca acagggcctc cacactgctc agccattttc
     4321 tcaaacacct tttcaaagta aaaacacaac aactcctttc ttgtggctgt tgctattctc
     4381 accctgtttt ttgcagccac ctgatcagca gcttgggtgc agatgttttt ctgggtttta
     4441 cagtgcaaaa acaccttagc atttgcatgg tgggggctgt gctgctgcca gtgcactttc
     4501 ccgccacttt gggtgttgca cttctggcaa cttccagggg gatttgcaaa ttccaaatac
     4561 attcccataa ttaacaatgg atctgtactg ttcagcacat tggcaaattc acaaagcttt
     4621 atccagtcaa cttctttgcc cttctgcttt tgctcatcaa aatcatagtc atgcaaaccc
     4681 ccctctttac tggcaggacc ccgggtaaaa gggggatctc ccaaggcatt ataacagtcc
     4741 tgttgtttta gcacagctct acaatggata aaggaagaag cacatatttt tctaccccaa
     4801 ttttgcacag cagtaaccct gtgtttgcca ggacatatca tatacaatat tgccccctct
     4861 acaccatcat taactttaga aatgcaccaa aacaaacaat taaattttct agtcatatgc
     4921 acttgcatat cattaacttt ggattctgta gtatagcata taaagcaact atgagtctta
     4981 ttcccaaaaa cagcatggct tacaaatcca agcaaagcac taggaatgtc ctcaggaggc
     5041 gggggtccct tgggcttctt tttaggggga gtggagaagc tgctggcttg gctggtccct
     5101 tcaccaggat ctgcagggtc ctcatcatca ctgcttggaa gctcttcatc acatctcagg
     5161 ttttcctcat cccaatcctt gttgaattct tcctgcaact ttcttgtaca aagttggcat
     5221 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     5281 aatcattatt tgccatccag ctgataagca agtaaaacct ctacaaatgt ggtaaaatcg
     5341 ataaggatcc gggctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5401 tgcgtaccta accaagttcc tctttcagag gttatttcag gccctgcagg ttaattaaca
     5461 aaaaaccccg ccggggcggg gttttttgtt acaattctta tcctcagtcc tgctcctctg
     5521 ccacaaagtg cacacagttg cctgctgggt ctctcagggc aaactctctc ccccagggct
     5581 gctctccaat ctctgtcatg gcagggccac tggcatccct gaagttggtg gagaccacct
     5641 cactccactc agcatacagc tcatccagtc ctctcaccca cacccaagcc agggtgttgt
     5701 caggcaccac ctggtcctgg actgctgaga tgaacagggt gacatcatct ctgaccacac
     5761 ctgcaaagtc atcctccaca aaatctctgg agaaccccaa cctgtcagtc cagaactcaa
     5821 cagctccagc cacatccctg gctgtgagca ctgggacagc actggtcaac ttggccatga
     5881 tggccctcct atagtgagtc gtattatact atgccgatat actatgccga tgattaattg
     5941 tcaactactt ttgaaaaatt taattaagaa catgtgagca aaaggccagc aaaaggccag
     6001 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6061 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6121 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6181 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6241 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6301 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6361 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6421 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6481 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6541 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6601 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6661 gaacgaaaac tcacgttaag ggattttggt catggctagt taattaaccc acgtttcaac
     6721 atgcacatcc cagtaatttg gaaacatttt gtttccaaag attcacttaa cattggttta
     6781 gcaacatgaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
     6841 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
     6901 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
     6961 ggtctcccca tgcgagagta gggaactgcc aggcatcaa
//
