LOCUS       sequence-102311-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Middle Entry Vector Containing QS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons 
            MJ, Halpern ME
  TITLE     Adoption of the Q transcriptional regulatory system for zebrafish 
            transgenesis.
  JOURNAL   Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. 
            Epub 2013 Jun 20.
  PUBMED    23792917
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2013.06.012"; journalName: "Methods"; date: 
            "2014-04-1- 1"; volume: "66"; issue: "3"; pages: "433-40"
COMMENT     Sequence Label: pME-QS (L1-L2)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    106..240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(130..149)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     184..203
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(512..532)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          662..727
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
       61 tcttataatg ccaactttgt acaagaaagc tgggtgcctg gatccgatcc agacatgata
      121 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      181 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      241 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      301 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
      361 tatacatcaa atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg
      421 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta
      481 gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag
      541 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca
      601 ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa
      661 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta
      721 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga
      781 agtaaggttc cttcacaaag atcctctagg gccgctcaag atatttgcgt tgcaattccg
      841 ttgaggcgag gctgtaactg cgcggtatga gattttgctt gatcatctgg gtatgctcgc
      901 aaaa
//
