LOCUS       sequence-102312-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' Gateway Entry Clone Containing 4xnrUAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Akitake CM, Macurak M, Halpern ME, Goll MG
  TITLE     Transgenerational analysis of transcriptional silencing in 
            zebrafish.
  JOURNAL   Dev Biol. 2011 Apr 15;352(2):191-201. doi: 
            10.1016/j.ydbio.2011.01.002. Epub 2011 Jan 9.
  PUBMED    21223961
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2011.01.002"; journalName: "Dev Biol"; date: 
            "2011-04-15- 15"; volume: "352"; issue: "2"; pages: "191-201"
COMMENT     Sequence Label: p5E-4xnrUAS (L4-R1)
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     149..165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     149..165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     170..189
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        170..188
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(261..384)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(561..656)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(696..713)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(696..712)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(705..727)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      776..862
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
       61 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
      121 gagattttga gacacgggcc agagctgcca ggaaacagct atgaccatgt aatacgactc
      181 actatagggg atatcgcggc cgccctgcag ctctagagct cgaattctac aggtcactaa
      241 taccatctaa gtagttggtt catagtgact gcatatgttg tgttttacag tattatgtag
      301 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      361 gttcaacttt tttgtacaaa cttggtgtgg aggagctcaa agtgaggctg agacgcgatg
      421 ggatccatta tataccctct agagtcgacc cttgcacgcg tcggaggaca gtactccgag
      481 gaaccctacg gaagactctc ctccgcctgt gaagacggag ggctgtcacc cgctaggact
      541 cacggattag aagccaccgt caacttttct atacaaagtt ggcattataa aaaagcattg
      601 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttggag
      661 ctccatggta gcgttaactc gggcccgagc ttaagactgg ccgtcgtttt acaacgtcgt
      721 gactgggaaa acatccatgc tagcgttaac gcgagagtag ggaactgcca ggcatcaaat
      781 aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      841 cgctctcctg agtaggacaa atccgc
//
