LOCUS       sequence-102314-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' Gateway Entry Vector Containing QUAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons 
            MJ, Halpern ME
  TITLE     Adoption of the Q transcriptional regulatory system for zebrafish 
            transgenesis.
  JOURNAL   Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. 
            Epub 2013 Jun 20.
  PUBMED    23792917
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2013.06.012"; journalName: "Methods"; date: 
            "2014-04-1- 1"; volume: "66"; issue: "3"; pages: "433-40"
COMMENT     Sequence Label: p5E-QUAS (L4-R1)
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     150..166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     150..166
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     171..190
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        171..189
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(262..385)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(577..672)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(712..729)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(712..728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(721..743)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      792..863
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
       61 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
      121 agagattttg agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact
      181 cactataggg gatatcgcgg ccgccctgca gctctagagc tcgaattcta caggtcacta
      241 ataccatcta agtagttggt tcatagtgac tgcatatgtt gtgttttaca gtattatgta
      301 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
      361 cgttcaactt ttttgtacaa acttgacgcg tcttcgaggt cgagggaatt cgtgtggagg
      421 agctcaaagt gaggctgaga cgcgatggga tccattatat accctctaga gtcgaccctt
      481 gcaaggataa gcgattaccc gaggataagc gattacccga gcggataagc gattacccgt
      541 gaggataatt gtttatccga ggataagcga ttaccccaac ttttctatac aaagttggca
      601 ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
      661 aaatcattat ttggagctcc atggtagcgt taactcgggc ccgagcttaa gactggccgt
      721 cgttttacaa cgtcgtgact gggaaaacat ccatgctagc gttaacgcga gagtagggaa
      781 ctgccaggca tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt cgttttatct
      841 gttgtttgtc ggtgaacgct ctcct
//
