LOCUS       sequence-102317-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen bicistronic lentiviral vector allows for simultaneous 
            expression of a transgene and EGFP marker to facilitate tracking of 
            transduced cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(105..121)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(106..122)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    135..723
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(188..208)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(606..617)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(867..900)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgactagct agtagctagc
       61 tagctagtcg agctcaagct tcgaattcga tatcaagctt atcgcgatac cgtcgacctc
      121 gagggaattc cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
      181 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
      241 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
      301 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
      361 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
      421 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
      481 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
      541 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
      601 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
      661 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
      721 cgcatcggga attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
      781 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
      841 caagatggga tcaattcacc atgggaataa cttcgtatag catacattat acgaagttat
      901 gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
      961 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     1021 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     1081 agtgtggaaa
//
