LOCUS       sequence-102318-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen bicistronic lentiviral vector allows for simultaneous 
            expression of a transgene and EGFP marker to facilitate tracking of 
            transduced cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(145..147,148..150,151..861)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(145..147,148..150,151..861)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(190..211)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(451..470)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     798..819
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(940..956)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(941..957)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1023..1043)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
       61 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
      121 tttgaaaaac acgatgataa taccatggtg agcaagggcg aggagctgtt caccggggtg
      181 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      241 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      301 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      361 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      421 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      481 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      541 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      601 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      661 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      721 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      781 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      841 ggcatggacg agctgtacaa gtccggactc agatctcgac tagctagtag ctagctagct
      901 agtcgagctc aagcttcgaa ttcgatatca agcttatcgc gataccgtcg acctcgaggg
      961 aattccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     1021 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     1081 gcttcccg
//
